The rapid and sensitive detection of pathogenic bacteria is very important for timely prevention and treatment of foodborne disease. Here, a bacteria-imprinted conductive poly(3-thiopheneacetic acid) (BICP) film-based impedimetric sensor was developed for the rapid, sensitive and label-free detection of staphylococcus aureus (S. aureus). The BICP film preparation was very convenient and eco-friendly, which was in situ deposited on gold electrode surface without the use of toxic organic solvents and cross-linkers. The process of imprinting and recognition were characterized by electrochemical technique and scanning electron microscope. The BICP had a novel structure without cocci-shaped cavities formed in the poly(3-thiopheneacetic acid) (PTAA) matrices. To obtain the optimal sensing performance, a set of factors affecting the imprinting and recognition were investigated. Under the optimized conditions, an extremely rapid recognition within 10 min, a very low limit of detection (LOD) of 2 CFU/mL, and wide linear range from 10 to 10⁸ CFU/mL were achieved by the BICP film-based impedimetric sensor. The sensor also demonstrated high selectivity, and good universality and repeatability. Furthermore, the feasibility of its application has also been demonstrated in the analysis of real milk samples. This sensor offered a simple and universal method for rapid, sensitive, and selective detection of pathogenic bacteria, which could hold great potentials in fields like food safety.

快速，灵敏地检测病原细菌对于及时预防和治疗食源性疾病非常重要。在这里，开发了一种基于细菌印迹的导电聚（3-噻吩乙酸）（BICP）膜的阻抗传感器，用于快速，灵敏且无标签的金黄色葡萄球菌（S. aureus）检测）。BICP膜的制备非常方便且环保，无需使用有毒的有机溶剂和交联剂即可原位沉积在金电极表面。通过电化学技术和扫描电子显微镜对印迹和识别过程进行了表征。BICP具有新颖的结构，在聚（3-噻吩乙酸）（PTAA）基质中没有形成球状腔。为了获得最佳的感测性能，研究了影响印记和识别的一系列因素。在优化的条件下，可在10分钟内快速识别，检测限（LOD）仅为2 CFU / mL，线性范围从10到10 ⁸ 通过基于BICP膜的阻抗传感器获得CFU / mL。该传感器还显示出高选择性，良好的通用性和可重复性。此外，在实际牛奶样品的分析中也证明了其应用的可行性。该传感器为快速，灵敏和选择性地检测病原细菌提供了一种简单而通用的方法，这种方法在食品安全等领域具有巨大的潜力。