Lepidoptera is the second most diverse insect order outnumbered only by the Coeleptera. Acetylcholinesterase (AChE) is the major target site for insecticides. Extensive use of insecticides, to inhibit the function of this enzyme, have resulted in the development of insecticide resistance. Complete knowledge of the target proteins is very important to know the cause of resistance. Computational annotation of insect acetylcholinesterase can be helpful for the characterization of this important protein. Acetylcholinesterase of fourteen lepidopteran insect pest species was annotated by using different bioinformatics tools. AChE in all the species was hydrophilic and thermostable. All the species showed lower values for instability index except L. orbonalis, S. exigua and T. absoluta. Highest percentage of Arg, Asp, Asn, Gln and Cys were recorded in P. rapae. High percentage of Cys and Gln might be reason for insecticide resistance development in P. rapae. Phylogenetic analysis revealed the AChE in T. absoluta, L. orbonalis and S. exigua are closely related and emerged from same primary branch. Three functional motifs were predicted in eleven species while only two were found in L. orbonalis, S. exigua and T. absoluta. AChE in eleven species followed secretory pathway and have signal peptides. No signal peptides were predicted for S. exigua, L. orbonalis and T. absoluta and follow non secretory pathway. Arginine methylation and cysteine palmotylation was found in all species except S. exigua, L. orbonalis and T. absoluta. Glycosylphosphatidylinositol (GPI) anchor was predicted in only nine species.

鳞翅目是第二大多样性昆虫类，仅次于鞘翅目。乙酰胆碱酯酶（AChE）是杀虫剂的主要目标部位。广泛使用杀虫剂来抑制这种酶的功能，导致了对杀虫剂的抗性的发展。全面了解靶蛋白对于了解耐药原因非常重要。昆虫乙酰胆碱酯酶的计算注释可有助于表征这一重要蛋白质。使用不同的生物信息学工具对14种鳞翅目害虫的乙酰胆碱酯酶进行了注释。在所有物种中，AChE具有亲水性和热稳定性。除L. orbonalis，S。exigua和T. absoluta外，所有物种的不稳定性指数均较低。。在菜青虫中记录到最高的Arg，Asp，Asn，Gln和Cys百分比。半胱氨酸和谷氨酸的高含量可能是菜青虫产生抗药性的原因。进化分析表明乙酰胆碱酯酶的T. absoluta，L. orbonalis和甜菜夜蛾有密切的关系，并从相同的主分支出现了。在11个物种中预测了3个功能性基序，而在兰色梭菌，S。exigua和T. absoluta中仅发现了2个。11种物种中的AChE遵循分泌途径并具有信号肽。没有预测到S. exigua，L。orbonalis和T. absoluta的信号肽并遵循非分泌途径。除S. exigua，L. orbonalis和T. absoluta以外的所有物种中都发现精氨酸甲基化和半胱氨酸棕榈酸酯化。仅在9个物种中预测了糖基磷脂酰肌醇（GPI）锚。