By using the indigenous micro-organisms of the polluted environment to be treated, bioremediation can be a successful strategy. PCR and RT-PCR molecular techniques were applied to examine the evolution of fungal isolates through putative genes f ligninolytic enzymes like lignin peroxidase (LiP), laccase (LaC), manganese peroxidase (MnP), and cellulase (Cx) as a response to polluting of the environment by hydrocarbons. In this study, isolation of rhizospheric fungal isolates, molecular identification, crude oil tolerance, and enzyme excretions were demonstrated. From the date palm rhizosphere, 3 fungal isolates were isolated and characterized morphologically and molecularly by ITS ribosomal RNA (rRNA) sequencing. The isolates were identified as Aspergillus flavus AF15, Trichoderma harzianum TH07, and Fusarium solani FS12 through using the BLAST tool in NCBI. All fungal isolates showed high tolerance to crude oil and survived with various responses at the highest concentration (20%). Aspergillus flavus AF15 and Trichoderma harzianum TH07 demonstrated promising oil-degrading tolerance ability based on the dose inhibition response percentage (DIRP) of the fungal isolates. A. flavus had a powerful capacity to production Cx, LaC, LiP and MnP with a range from 83.7 to 96.3 mL. Molecularly, nine genes of the ligninolytic enzymes, cbh (cbhI.1, cbhI.1, cbhII) lcc, lig (1, 2, 4 and 6) and mnp were tested for presence and expression (by PCR and RT-PCR, respectively). PCR showed that all isolates contained all the nine genes examined, regardless of capacity to enzymes production profiles, so the presence responses of nine genes did not correlate with enzymes-production ability. Gene expression analysis shows a more diverse pattern for tested isolates for example, Aspergillus flavus AF15 had over-expression of lig and mnp genes, Fusarium solani FS12 have a weak signal with lcc gene while, Trichoderma harzianum TH07 showed moderate expression of mnp and lcc genes. The power of the transcription of the gene leads to increased enzyme secretion by fungal isolates. Fungi are important microorganisms in the clean-up of petroleum pollution. They have bioremediation highly potency that is related to their diverse production of these catalytic enzymes.

通过利用受污染环境中的本土微生物进行处理，生物修复可以成为一种成功的策略。应用 PCR 和 RT-PCR 分子技术来检查真菌分离株通过假定基因 f 木质素分解酶（如木质素过氧化物酶（LiP）、漆酶（LaC）、锰过氧化物酶（MnP）和纤维素酶（Cx））的进化，作为对污染的反应碳氢化合物对环境的影响。在这项研究中，展示了根际真菌分离株的分离、分子鉴定、原油耐受性和酶排泄。从枣椰树根际分离出 3 种真菌分离株，并通过 ITS 核糖体 RNA (rRNA) 测序进行形态学和分子特征鉴定。利用NCBI的BLAST工具将分离菌株鉴定为黄曲霉AF15、哈茨木霉TH07和茄镰刀菌FS12。所有真菌分离株均对原油表现出高度耐受性，并在最高浓度（20%）下以各种反应存活。根据真菌分离株的剂量抑制反应百分比 (DIRP)，黄曲霉AF15 和哈茨木霉TH07 表现出良好的油降解耐受能力。黄曲霉具有很强的Cx、LaC、LiP和MnP生产能力，产量范围为83.7～96.3 mL。在分子水平上，测试了木质素分解酶的 9 个基因cbh ( cbhI.1 、 cbhI.1 、 cbhII ) lcc 、 lig (1、2、4 和 6) 和mnp的存在和表达（分别通过 PCR 和 RT-PCR） ）。 PCR表明，无论酶生产能力如何，所有分离株都包含所检查的所有九个基因，因此九个基因的存在反应与酶生产能力无关。基因表达分析显示测试分离株的模式更加多样化，例如，黄曲霉AF15 过度表达lig和mnp基因， Fusarium solani FS12 具有lcc基因微弱信号，而哈茨木霉TH07 显示mnp和lcc基因中度表达。该基因转录的力量导致真菌分离株的酶分泌增加。真菌是净化石油污染的重要微生物。它们具有高效的生物修复能力，这与它们生产这些催化酶的多样性有关。