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Iso-seq analysis and functional annotation of the Santa Fe cave crayfish (Procambarus erythrops) transcriptome
Marine Genomics ( IF 1.3 ) Pub Date : 2021-01-21 , DOI: 10.1016/j.margen.2021.100842
Celine M Carneiro 1 , Jerald D Noble 2 , Adele Pietras 3 , Paul Moler 4 , James D Austin 1
Affiliation  

The genus Procambarus represents a diverse genus of freshwater crayfish that includes epigean species, stygobitic species, and at least one parthenogenic species. Despite its evolutionary, ecological, and economic importance, most genomic and transcriptomic resources for this genus are limited to a couple of model species. We sequenced the transcriptome of a non-model species, P. erythrops, a geographically restricted stygobitic species from Florida. RNA isolated from gill, muscle and eye tissue was pooled to create a de novo transcriptome assembly using Single Molecule Real-Time sequencing (PacBio), resulting in 19,442 full-length isoforms. The assembly has been deposited in the NCBI (BioProject PRJNA657230). These data will make an important contribution to the comparative study of transcriptome evolution in crayfish and crustaceans.



中文翻译:

Santa Fe洞穴小龙虾(Procambarus erythrops)转录组的Iso-seq分析和功能注释

Procambarus属代表了淡水小龙虾的多样化属,包括表生物种、stygobitic 物种和至少一种孤雌生殖物种。尽管它在进化、生态和经济方面具有重要意义,但该属的大多数基因组和转录组资源仅限于几个模式物种。我们对一种非模式物种P. erythrops的转录组进行了测序,这是一种来自佛罗里达州的地理上受限的 stygobitic 物种。从鳃、肌肉和眼组织中分离的 RNA 被汇集起来以创建一个从头使用单分子实时测序 (PacBio) 进行转录组组装,产生 19,442 个全长亚型。该组件已存放在 NCBI (BioProject PRJNA657230) 中。这些数据将为小龙虾和甲壳类动物转录组进化的比较研究做出重要贡献。

更新日期:2021-01-21
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