As antimicrobial resistance continues to threaten the efficacy of conventional antibiotic therapy, it is paramount that we investigate innovative approaches to treat infectious diseases. In this study, we investigated the antimicrobial capabilities of the innovative combination of antimicrobial blue light (aBL; 405 nm wavelength) with the Pseudomonas aeruginosa pigment pyocyanin against methicillin resistant Staphylococcus aureus (MSRA. We explored the effects of different radiant exposures of aBL and increasing concentrations of pyocyanin against planktonic cells and those within biofilms. In addition, we investigated the effect of the aBL/pyocyanin on the endogenous staphyloxanthin pigment, as well as the role of hydrogen peroxide and singlet oxygen scavenging in the efficacy of this combination. Lastly, we investigated the potential for the aBL/pyocyanin to reduce the MRSA burden within a proof-of-principle mouse abrasion infection model. We found pyocyanin to be a powerful potentiator of aBL activity under all in vitro conditions tested. In addition, we serendipitously discovered the capability of the aBL/pyocyanin combination to bleach staphyloxanthin within colonies of MRSA. Furthermore, we established that singlet oxygen is an important mediator during combined aBL/pyocyanin exposure. Moreover, we found that the combination of aBL and pyocyanin could significantly reduce the viability of MRSA within a proof-of-principle early onset MRSA skin abrasion infection. Exposure to the treatment did not have deleterious effects on skin tissue. In conclusion, the combination of aBL and pyocyanin represents a potentially powerful therapeutic modality for the treatment of infections caused by MRSA.

由于抗菌素耐药性继续威胁传统抗生素治疗的功效，因此我们研究治疗传染病的创新方法至关重要。在这项研究中，我们研究了抗菌蓝光（aBL；405 nm 波长）与绿脓杆菌色素绿脓素的创新组合对耐甲氧西林金黄色葡萄球菌的抗菌能力我们确定单线态氧是结合 aBL/绿脓素暴露期间的重要介质。此外，我们发现 aBL 和绿脓杆菌素的组合可以显着降低 MRSA 在原理验证性早发性 MRSA 皮肤磨损感染中的生存能力。暴露于治疗对皮肤组织没有有害影响。总之，aBL 和绿脓杆菌素的组合代表了一种用于治疗由 MRSA 引起的感染的潜在强大的治疗方式。