Introduction

Basophils play a major physio-pathological role in hypersensitivity related diseases. Basophils express high affinity Immunoglobulin (Ig) E receptors (FcεRI), IgG and complement regulatory. Basophils also have immunoregulatory activity through interaction with T cells. The aim of this study was to look for the expression of markers reflecting the activation status of peripheral Basophil in healthy donors.

Method

the study was performed on 29 healthy donors, 62% females with a mean age of 50.1 + 17.0 years. Basophils were identified on their expression of CD123 without HLA-DR and/or CD193 in two 8 colors panels including CD46, CD55, CD59, CD203c, CD32 (FcγRII), CD64 (FcγRIII), CD163, CD137L (4-1BBL), CD252 (OX40L), CD244 (2B4) and CD3 on whole blood. Basophil activation with anti IgE was performed on 14 donors.

Results and discussion

Our results confirmed the Basophil expression of CD123, CD193 and CD203 (the latter is strongly increased under stimulation). Complement regulatory proteins (CD46, CD55, CD59) were expressed at the same levels as on other leukocytes; CD46, CD59 expression being slightly increased under stimulation. CD32 and CD163 scavenger were slightly higher than on lympho and not influenced by activation. CD252 or CD137L were expressed at low levels and significantly induced by stimulation. Most of all, CD244 was highly expressed on Basophils as compared to any other leukocytes in fresh peripheral blood.

Conclusions

Our study shows that human resting Basophils express IgE and IgG Fc receptors and check point receptor CD244 that could potentially play a role in their previously reported immunoregulatory activity in sensitization and even in tumor immune escape.

中文翻译：

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人外周嗜碱性粒细胞扩展表型显示 CD244 免疫调节受体的高表达

介绍

嗜碱性粒细胞在超敏反应相关疾病中发挥重要的生理病理作用。嗜碱性粒细胞表达高亲和力免疫球蛋白 (Ig) E 受体 (FcεRI)、IgG 和补体调节。嗜碱性粒细胞还通过与 T 细胞相互作用而具有免疫调节活性。本研究的目的是寻找反映健康供体外周嗜碱性粒细胞活化状态的标志物的表达。

方法

该研究对 29 名健康捐献者进行，其中 62% 为女性，平均年龄为 50.1 + 17.0 岁。嗜碱性粒细胞在两个 8 色面板中的 CD123 表达上被鉴定，其中没有 HLA-DR 和/或 CD193，包括 CD46、CD55、CD59、CD203c、CD32 (FcγRII)、CD64 (FcγRIII)、CD163、CD137L (4-1BBL)、CD252 (OX40L)、CD244 (2B4) 和全血 CD3。用抗 IgE 对 14 位供体进行了嗜碱性粒细胞活化。

结果和讨论

我们的结果证实了 CD123、CD193 和 CD203 的嗜碱性粒细胞表达（后者在刺激下强烈增加）。补体调节蛋白（CD46、CD55、CD59）的表达水平与其他白细胞相同；CD46、CD59 表达在刺激下略有增加。CD32 和 CD163 清道夫略高于淋巴，不受激活的影响。CD252 或 CD137L 以低水平表达并受到刺激显着诱导。最重要的是，与新鲜外周血中的任何其他白细胞相比，CD244 在嗜碱性粒细胞上高度表达。

结论

我们的研究表明，人类静息嗜碱性粒细胞表达 IgE 和 IgG Fc 受体以及检查点受体 CD244，这可能在其先前报道的致敏甚至肿瘤免疫逃逸的免疫调节活性中发挥作用。