Herein, we report an artificial nanoenzyme: CoSe₂ with unique flake like morphology, exhibiting an excellent intrinsic peroxidase like activity. In comparison with naturally found Horseradish peroxidase (HRP), silver and gold based biomimetics, these nanoflakes mimics enhanced peroxidase like activity with higher K_cat and V_max values for oxidation of both 3,3,5,5-tetramethylbenzidine (K_cat = 42.8 s⁻¹, V_max = 0.5139 μM/s) and o-phenylenediamine (K_cat = 84.95 s⁻¹, V_max = 1.0139 μM/s) under optimized pH conditions. A single electron transfer mechanism from TMB/OPD substrate favors formation of blue color TMB⁺/OX-OPD products. The unique flakes like morphology of CoSe₂ nanoflakes provides active channels which offers better accessibility for the reactant, resulting in enhanced catalytic efficiency of CoSe₂ nanoflakes. Additionally, there is no significant loss in the catalytic efficiency of CoSe₂ nanoflakes even after 365 days which endorses higher thermal and chemical stability of these synthesized CoSe₂ nanoflakes.

在这里，我们报告了一种人工纳米酶：CoSe ₂具有独特的片状形态，表现出出色的内在过氧化物酶样活性。与天然存在的辣根过氧化物酶（HRP），基于银和金的仿生物相比，这些纳米薄片模拟了增强的过氧化物酶活性，具有较高的3,3,5,5-四甲基联苯胺的K _cat和V _max值（K _cat  = 42.8） s ^-1，V _max  = 0.5139μM/ s）和邻苯二胺（K _cat  = 84.95 s ^-1，V _max = 1.0139μM/ s）在优化的pH条件下。来自TMB / OPD基板的单一电子转移机制有利于形成蓝色TMB ⁺ / OX-OPD产品。独特的薄片（如CoSe ₂纳米薄片的形态）提供了活性通道，为反应物提供了更好的可及性，从而提高了CoSe ₂纳米薄片的催化效率。另外，即使在365天后，CoSe ₂纳米薄片的催化效率也没有显着损失，这证明了这些合成的CoSe ₂纳米薄片的更高的热和化学稳定性。