A fluorescent Au nanocluster LRL−AuNCs modified with RGDK-containing peptide was synthesized with a simple one-pot method. Based on the “turn-off” model, LRL−AuNCs displayed high sensitivity and selectivity for sensing Cu²⁺. It showed a good linear calibration between the fluorescence intensity and Cu²⁺ concentration in the range of 5 ~ 80 μM (R² = 0.998), with the detection limit 3.9 μM. which is far lower than the maximum allowable concentration of Cu²⁺ (20 μM) in drinking water. Therefore, the detection of Cu²⁺ in water sample was performed in a handy way. As LRL−AuNCs showed low toxicity and good biocompatibility, LRL−AuNCs was used to sense the change of Cu²⁺ level in tumor cells by two-photon photoluminescence (TPPL) imaging. These results indicated that the LRL−AuNCs as a TPPL probe showed potential in tumor diagnosis and treatment.

用简单的一锅法合成了用含RGDK的肽修饰的荧光金纳米簇LRL-AuNCs。基于“关闭”模型，LRL-AuNCs对感测Cu ²⁺具有很高的灵敏度和选择性。在5〜80μM（R ²  = 0.998）的范围内，荧光强度与Cu ²⁺浓度之间具有良好的线性校准，检出限为3.9μM。远低于饮用水中的最大允许浓度Cu ²⁺（20μM）。因此，以简便的方式进行了水样品中Cu ²⁺的检测。由于LRL-AuNCs具有低毒性和良好的生物相容性，因此LRL-AuNCs可用于检测Cu ²⁺的变化。双光子光致发光（TPPL）成像检测肿瘤细胞中的血红蛋白水平。这些结果表明，作为TPPL探针的LRL-AuNCs在肿瘤的诊断和治疗中具有潜力。