The comparative transcriptome analysis of the fungus Gibberella zeae which could efficiently catalyze the 7β-hydroxylation of LCA to produce UDCA was performed with LCA induction. This is the first time to report the comparative transcriptome of fungus under LCA treatment. Totally, 1364 differentially expressed genes including 770 up-regulated and 594 down-regulated genes were identified. In the 770 up-regulated genes, 12 genes with the function of hydroxylation were picked out by application of function screening, which were annotated as CYP450 or hydroxylase. Moreover, the qRT-PCR results of five up-regulated CYP450-like genes confirmed the credibility of RNA-Seq further. These results provide valuable information for the discovery of novel enzyme producing clinical drug UDCA from butchery byproduct LCA, and also might indicate some clues for the detoxification process of LCA in humans.

中文翻译：

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真菌玉米赤霉将石胆酸转化为熊去氧胆酸的转录组比较分析

通过LCA诱导，对能有效催化LCA的7β-羟基化产生UDCA的真菌玉米赤霉进行比较转录组分析。这是首次报道了 LCA 处理下真菌的比较转录组。共鉴定出1364个差异表达基因，包括770个上调基因和594个下调基因。在770个上调基因中，应用功能筛选筛选出12个具有羟化功能的基因，注释为CYP450或羟化酶。此外，五个上调的 CYP450 样基因的 qRT-PCR 结果进一步证实了 RNA-Seq 的可信度。这些结果为从屠宰副产品 LCA 中发现新的产酶临床药物 UDCA 提供了有价值的信息，