OBJECTIVES To screen for a variety of marine bacteria with anti-quorum sensing and anti-biofilm activities. RESULTS Among 188 bacterial isolates from water, sediment, and corals in the Red Sea region, approximately 35% (65 isolates) of the isolates displayed a significant degradation in the purple pigment of the bioreporter strain without affecting cell growth. The quorum quenching bacteria obtained from coral-associated bacteria were 66.2% out of the total isolates. The PCR amplification results revealed that the recorded Acyl Homoserine lactone (AHL) inhibition by 91% of the anti-QS marine bacteria was not due to lactonase activity. On the other hand, lactonase genes were recorded only in the remaining 9% (6 isolates) and those were belonging to genus Bacillus, Nocardiopsis, and Enterobacter based on 16S rRNA gene sequences. The results also showed that marine bacteria with anti-QS activity inhibited 67% of the biofilm formed by Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio alginolyticus. The computational profiling analysis confirmed the presence of the functional region in the detected genes. CONCLUSION Coral microbial communities are rich sources for pharmacologically important natural products with anti-quorum sensing and anti-biofilm activities.

中文翻译：

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从埃及不同海洋资源中分离出的一些海洋细菌的反群体感应活动

目的筛选具有抗群体感应和抗生物膜活性的多种海洋细菌。结果 在红海地区水、沉积物和珊瑚的 188 株细菌分离物中，大约 35%（65 株）的分离株显示出生物报告菌株紫色色素的显着降解，而不影响细胞生长。从珊瑚相关细菌中获得的群体淬灭细菌占总分离株的 66.2%。PCR 扩增结果显示，91% 的抗 QS 海洋细菌所记录的酰基高丝氨酸内酯 (AHL) 抑制作用不是由于内酯酶活性。另一方面，仅在剩余的 9%（6 个分离株）中记录了内酯酶基因，根据 16S rRNA 基因序列，这些基因属于芽孢杆菌属、诺卡氏菌属和肠杆菌属。结果还表明，具有抗QS活性的海洋细菌抑制了67%的由嗜水气单胞菌、铜绿假单胞菌和溶藻弧菌形成的生物膜。计算图谱分析证实了检测到的基因中存在功能区。结论 珊瑚微生物群落是具有抗群体感应和抗生物膜活性的药理学重要天然产物的丰富来源。