Bacteriocins are proteinaceous compounds produced from lactic acid bacteria. Bacteriocins are well-known for their antibacterial potential and safety for application in food. However, the commercial availability of bacteriocin is facing several limitations; among them is the low yield and short stability period. That calls for a new strategy for overcoming these hurdles. Among these approaches is incorporating bacteriocin in nanoparticles. So, the aim of this study was to enhance the plantaricin produced from isolated Lactobacillus plantarum strain using nanotechnology. In this study, the plnEF genes encoding plantaricin EF have been identified and sequenced (accession number of MN172264.1). The extracted bacteriocin (EX-PL) was obtained by the ammonium sulfate method. Then, it was used for biosynthesizing plantaricin-incorporated silver nanoparticles (PL-SNPs). The synthesized nanoparticles were confirmed by SEM-EDAX analysis. The antibacterial activity of both combined (PL-SNPs) and extracted plantaricin (EX-PL) were tested against some strains of foodborne pathogenic bacteria. The results revealed that the antibacterial activities were increased by 99.2% on the combination of bacteriocin with the silver nanoparticle. The MIC of EX-PL (7.6 mg/mL) has been lowered after incorporating into silver nanoparticles and reached 0.004 mg/mL for PL-SNPs. Despite that extracted plantaricin showed no inhibitory activity towards Listeria monocytogenes, plantaricin-incorporated silver nanoparticles displayed inhibitory activity against this strain. Furthermore, the stability period at 4 °C was increased from 5 days to 60 days for EX-PL and PL-SNPs, respectively. Plantaricin-incorporated silver nanoparticles possess higher antibacterial activity and more stability than the free one, which makes it more fitting for combating foodborne pathogens and open more fields for applications in both food and pharmaceutical industries.

中文翻译：

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通过掺入银纳米颗粒增强plant草素的抗菌潜能

细菌素是从乳酸菌产生的蛋白质化合物。细菌素以其抗菌潜力和在食品中使用的安全性而闻名。然而，细菌素的商业可获得性面临一些限制。其中产量低，稳定期短。这就要求采取新的战略来克服这些障碍。在这些方法中，将细菌素掺入纳米颗粒中。因此，本研究的目的是使用纳米技术增强从分离的植物乳杆菌菌株中产生的plant藤素。在这项研究中，已鉴定并编码了编码plant藤素EF的plnEF基因（登录号为MN172264.1）。提取的细菌素（EX-PL）通过硫酸铵法获得。然后，它用于生物合成掺入plant藤素的银纳米颗粒（PL-SNP）。通过SEM-EDAX分析确认了合成的纳米颗粒。联合（PL-SNPs）和提取的车瑞霉素（EX-PL）的抗菌活性均对某些食源性致病细菌菌株进行了测试。结果表明，细菌素与银纳米颗粒的组合，抗菌活性提高了99.2％。掺入银纳米颗粒后，EX-PL的MIC（7.6 mg / mL）降低，PL-SNPs的MIC达到0.004 mg / mL。尽管提取的植物霉素对单核细胞增生李斯特菌没有抑制活性，但结合有植物霉素的银纳米颗粒对该菌株却具有抑制活性。此外，EX-PL和PL-SNP的4°C稳定期分别从5天增加到60天。掺入藤霉素的银纳米颗粒比游离的银纳米颗粒具有更高的抗菌活性和稳定性，这使其更适合于对抗食源性病原体，并为食品和制药行业开辟了更多领域。