This research aimed at investigating the hypoglycemic effect of Zanthoxylum alkylamides and whether TRPV1 receptor could participate in the glucose metabolism by using streptozotocin-induced diabetic rat model. The results showed that the blood glucose measured in the Zanthoxylum alkylamides treated group (ALK) showed significantly lower values than that in the model group (Model). Significant improvements in the oral glucose tolerance as well as plasma insulin and hepatic glycogen were also observed in the ALK group, when compared to the model group. However, the improving effects of Zanthoxylum alkylamides on glucose metabolism disorder in diabetic rats were markedly inhibited by capsazepine as the TRPV1 receptor antagonist. In addition, there were significant differences in the levels of mRNA and protein of phosphoenolpyruvate carboxylase (PEPCK), gucose-6-phosphatase (G6Pase), glucokinase (GK) and cannabinoid receptor l (CB1) in the livers of the ALK group compared to model group. Meanwhile, ALK group also exhibited a remarkable increase in the pancreatic-duodenal homeobox 1 (PDX-1), glucose transporter 2 (GLUT 2), GK levels and a significant decrease in the expression levels of CB1 in the pancreas, while the presence of capsazepine would affected the expression of these genes. These findings indicate that Zanthoxylum alkylamides could ameliorate the glucose metabolism disorder in diabetic rats. Furthermore, the TRPV1 receptor could participate in regulating the expressions of genes and proteins related to glucose metabolism and insulin secretion in the liver and pancreas, and takes a role in the hypoglycemic process of Zanthoxylum alkylamides.

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花椒烷基酰胺对链脲佐菌素诱导的糖尿病Sprague-Dawley大鼠葡萄糖代谢的影响

本研究旨在通过链脲佐菌素诱导的糖尿病大鼠模型研究花椒烷基酰胺的降血糖作用以及TRPV1受体是否可以参与葡萄糖代谢。结果表明，在花椒烷基酰胺处理组（ALK）中测得的血糖值明显低于模型组（模型）。与模型组相比，ALK组还观察到口服葡萄糖耐量以及血浆胰岛素和肝糖原的显着改善。然而，辣椒素作为TRPV1受体拮抗剂明显抑制了花椒烷基酰胺对糖尿病大鼠葡萄糖代谢紊乱的改善作用。此外，与模型组相比，ALK组肝脏中磷酸烯醇丙酮酸羧化酶（PEPCK），葡萄糖6磷酸酶（G6Pase），葡萄糖激酶（GK）和大麻素受体1（CB1）的mRNA和蛋白水平存在显着差异。同时，ALK组在胰腺十二指肠同源盒1（PDX-1），葡萄糖转运蛋白2（GLUT 2），GK水平和胰腺中CB1的表达水平显着降低，同时还存在胰腺癌。辣椒素会影响这些基因的表达。这些发现表明，花椒烷基酰胺可以改善糖尿病大鼠的葡萄糖代谢紊乱。此外，TRPV1受体可以参与调节肝脏和胰腺中与葡萄糖代谢和胰岛素分泌有关的基因和蛋白质的表达，