Background: Sporadic Alzheimer’s Disease (AD) is assumed to be associated with different biological/genetic vulnerability, as well as with neuroinflammation, mediated by cytokines. The present study evaluated the role of cytokines in AD.

Objective: The aim was to determine the possible association of TNF-α (rs1800629), IL1-α (rs1800587) and IL-10 (rs1800896) polymorphisms with AD, and to assess serum TNF-α, IL-1α and IL-10 concentrations in patients with AD and in subjects with mild cognitive impairment (MCI).

Methods: The study included 645 Caucasian participants: 395 subjects with AD and 250 subjects with MCI. Genotyping was performed using real-time PCR in all 645 subjects, while serum concentrations of TNF-α, IL-1α and IL-10 and were determined using ELISA in 174 subjects.

Results: The frequency of the TNF-α rs1800629, IL1-α rs1800587 or IL-10 rs1800896 genotypes did not differ significantly between patients with AD and MCI. Serum concentration of IL-1α and IL-10 were significantly decreased, while the concentration of TNF-α was significantly higher in patients with AD than in MCI subjects. TNF-α, IL1-α or IL-10 concentrations were similar in subjects with AD or MCI subdivided into carriers of the corresponding TNF-α rs1800629, IL1-α rs1800587 or IL-10 rs1800896 genotypes.

Conclusion: Similar distribution of the IL1-α rs1800587, TNF-α rs1800629 or IL-10 rs1800896 genotypes in subjects with AD and MCI failed to confirm that these specific risk genotypes are associated with vulnerability to develop AD. Alteration in IL-1α, IL-10 and TNF-α concentrations in patients with AD partially confirmed the association with the neuroinflammatory response in AD.

背景：散发性阿尔茨海默病 (AD) 被认为与不同的生物/遗传脆弱性以及由细胞因子介导的神经炎症有关。本研究评估了细胞因子在 AD 中的作用。

目的：旨在确定 TNF-α (rs1800629)、IL1-α (rs1800587) 和 IL-10 (rs1800896) 多态性与 AD 的可能关联，并评估血清 TNF-α、IL-1α 和 IL-10 AD 患者和轻度认知障碍 (MCI) 受试者中的浓度。

方法：该研究包括 645 名白人参与者：395 名患有 AD 的受试者和 250 名患有 MCI 的受试者。在所有 645 名受试者中使用实时 PCR 进行基因分型，同时在 174 名受试者中使用 ELISA 确定 TNF-α、IL-1α 和 IL-10 的血清浓度。

结果： TNF-α rs1800629、IL1-α rs1800587 或 IL-10 rs1800896 基因型的频率在 AD 和 MCI 患者之间没有显着差异。与MCI受试者相比，AD患者血清IL-1α和IL-10浓度显着降低，而TNF-α浓度显着升高。细分为相应 TNF-α rs1800629、IL1-α rs1800587 或 IL-10 rs1800896 基因型携带者的 AD 或 MCI 受试者的 TNF-α、IL1-α 或 IL-10 浓度相似。

结论： IL1-α rs1800587、TNF-α rs1800629 或 IL-10 rs1800896 基因型在 AD 和 MCI 受试者中的相似分布未能证实这些特定风险基因型与发生 AD 的易感性相关。AD 患者中 IL-1α、IL-10 和 TNF-α 浓度的变化部分证实了与 AD 中神经炎症反应的关联。