Cryptosporidium spp. is responsible for several food and waterborne disease outbreaks worldwide. Healthier lifestyles attract consumers to eat, notably, fresh food like fruits and vegetables. The consumption of raw or under-cooked food increases the risk of foodborne transmission of Cryptosporidiosis. The assessment of the consumer’s exposure to Cryptosporidium danger is crucial for public health. Still, the standardized method to detect this parasite in fresh leafy greens and berry fruits has only been available since 2016 and suffers from weaknesses. Consequently, in this study, we propose a method with minimum processing steps that combines cell culture and the quantitative PCR (CC–qPCR) for detecting infectious C. parvum oocysts recovered from lamb’s lettuce. This CC–qPCR is a rapid and easy method that can detect up to one oocyst, whereas it is undetectable by classic qPCR.

中文翻译：

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羔羊生菜中传染性小隐孢子虫卵囊的检测：CC-qPCR的摄入量

隐孢子虫属。导致全球数次食物和水传播疾病爆发。健康的生活方式会吸引消费者进食，尤其是水果和蔬菜等新鲜食品。生食或未煮熟食品的食用会增加隐孢子虫病通过食源传播的风险。对消费者暴露于隐孢子虫危害的评估对于公共卫生至关重要。尽管如此，自2016年以来仅提供了用于检测新鲜绿叶蔬菜和浆果中这种寄生虫的标准化方法，并且存在弱点。因此，在这项研究中，我们提出了一种处理步骤最少的方法，该方法结合了细胞培养和定量PCR（CC-qPCR）来检测传染性小球藻。从羔羊的生菜中回收的卵囊。这种CC-qPCR是一种快速简便的方法，可以检测多达一个卵囊，而传统的qPCR无法检测到。