Abstract

Two strains of the chlorophyte Chlamydomonas reinhardtii, a wild type (WT) and a transgenic strain (C.CYN) contained an exogenous cyanase gene (CYN), were used to investigate the growth and cyanate biosorption capability through the analysis of the adsorption equilibrium isotherm. The potential antioxidants activity of the algal strains was also investigated under cyanate concentration. The antioxidants activity of both C.CYN and WT were enhanced by the application of cyanate.

Two adsorption isotherm models and the sorption kinetics were used to check the efficiency of the cyanate removal process. The results showed the biosorbent efficiency of Chlamydomonas in the removal of KCNO from aqueous solution. The C.CYN strain has great efficiency to remove cyanate as compared to the WT. The maximum percentage of cyanate removal was 83.75% for the C.CYN and 50% for the WT as treated with 0.8 mg.ml⁻¹ KCNO. The data were adapted to the nonlinear Langmuir model on the basis of the coefficient of determination. The calculated q_max was 0.54 and 0.42 µg.mg⁻¹ for C.CYN and WT which correlated to the experimental one (0.67 and 0.4 µg.mg⁻¹, respectively). Our data highlight the application of the transgenic algal strain toward the removal of highly toxic materials as cyanate.

Novelty statement The main objective of this work is to find out an efficient genetically-modified Chlamydomonas strain to remove the highly toxic cyanate compound from contaminated area. Moreover, to evaluate the biosorption ability of this transgenic strain with its wild one via two adsorption isotherm (the Langmuir and Freundlich) models. Also, to estimate the antioxidants activity of these strains under the cyanate toxicity through four different assays.

摘要

使用含有外源氰化酶基因（CYN）的两株叶绿素莱茵衣藻，野生型（WT）和转基因株（C.CYN），通过吸附平衡等温线分析研究其生长和氰酸盐生物吸附能力。 . 还在氰酸盐浓度下研究了藻类菌株的潜在抗氧化活性。C.CYN 和 WT 的抗氧化活性通过氰酸盐的应用增强。

两个吸附等温线模型和吸附动力学用于检查氰酸盐去除过程的效率。结果表明衣藻从水溶液中去除 KCNO的生物吸附效率。与 WT 相比，C.CYN 菌株在去除氰酸盐方面具有很高的效率。用 0.8 mg.ml ^-1 KCNO处理的 C.CYN 和 WT 的最大氰酸盐去除百分比分别为 83.75% 和 50% 。数据根据决定系数适应非线性朗缪尔模型。对于 C.CYN 和 WT，计算出的 q _{max 分别}为 0.54 和 0.42 µg.mg ^-1，与实验相关（0.67 和 0.4 µg.mg ^-1， 分别）。我们的数据突出了转基因藻类菌株在去除剧毒物质如氰酸盐方面的应用。

新颖性声明这项工作的主要目的是找出一种有效的转基因衣藻菌株，以去除污染区域中剧毒的氰酸盐化合物。此外，通过两个吸附等温线（Langmuir 和 Freundlich）模型评估这种转基因菌株与其野生菌株的生物吸附能力。此外，通过四种不同的测定来估计这些菌株在氰酸盐毒性下的抗氧化活性。