Abstract

Diamondback moth is one of the destructive insect pests of brassicaceous vegetable crops worldwide with increased insecticide resistance. Chlorpyrifos is one of the most widely-used organophosphate insecticides to control various pests including P. xylostella. The insecticide resistance in Plutella xylostella can be best managed by perceiving CPF resistance inheritance. We designed this experiment to evaluate CPF resistance's genetics of two genetically pure lines of P. xylostella, namely insensitive AChE1-carrying Kar-R resistant strain and Gu-S wild-type strain. A considerable difference was observed among LC₅₀ values of F1 (Kar-R♀ × Gu-S♂) and F1′ (Kar-R♂ × Gu-S♀) strains due to reciprocal crosses between each resistant strain and susceptible strains. The degree of dominance values were 0.58 and 0.75, respectively suggesting an autosomal and partially dominant resistance. The χ² goodness-of-fit test results and measuring the number of genes which segregate in CPF-resistant strain suggested that several genetic factors control the resistance.

摘要

小菜蛾是全世界黄铜科蔬菜作物中具有破坏性的害虫之一，其杀虫剂抗性增强。毒死rif是控制各种有害生物（包括小菜蛾）的最广泛使用的有机磷酸盐杀虫剂之一。小菜蛾对杀虫剂的抗性可以通过了解CPF抗性遗传来最好地控制。我们设计了这个实验，以评估小菜蛾两个纯净品系的CPF抗性遗传，即不敏感的携带AChE1的Kar-R抗性菌株和Gu-S野生型菌株。LC ₅₀之间观察到相当大的差异F1（Kar-R♀×Gu-S♂）和F1'（Kar-R♂×Gu-S♀）菌株的抗性值是由于每个抗性菌株和易感菌株之间的相互交叉引起的。优势度值分别为0.58和0.75，表明常染色体抗性和部分显性抗性。的χ ²测试结果并测量在分离CPF-抗性菌株建议几种遗传因素控制电阻的基因数拟合优度-。