ABSTRACT

Introduction: Over the past decade, loop-mediated isothermal amplification (LAMP) technology has played an important role in molecular diagnostics. Amongst numerous nucleic acid amplification assays, LAMP stands out in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions.

Areas covered: In this review, we outline the front-end LAMP design practices for point-of-care (POC) applications, including sample handling and various signal readout methodologies. Next, we explore existing LAMP technologies that have been validated with clinical samples in the field. We summarize recent work that utilizes reverse transcription (RT) LAMP to rapidly detect SARS-CoV-2 as an alternative to standard PCR protocols. Finally, we describe challenges in translating LAMP from the benchtop to the field and opportunities for future LAMP assay development and performance reporting.

Expert opinion: Despite the popularity of LAMP in the academic research community and a recent surge in interest in LAMP due to the COVID-19 pandemic, there are numerous areas for improvement in the fundamental understanding of LAMP, which are needed to elevate the field of LAMP assay development and characterization.

抽象的

简介：在过去的十年中，回路介导的等温扩增（LAMP）技术在分子诊断中发挥了重要作用。在众多的核酸扩增测定中，LAMP在样品到答案的时间，灵敏度，特异性，成本，稳健性和可及性方面脱颖而出，使其成为资源有限区域中可现场部署的诊断的理想选择。

涵盖的领域：在本文中，我们概述了即时医疗（POC）应用的前端LAMP设计实践，包括样本处理和各种信号读取方法。接下来，我们探索现有的LAMP技术，该技术已在该领域的临床样品中得到验证。我们总结了利用逆转录（RT）LAMP快速检测SARS-CoV-2作为标准PCR方案的替代方法的最新工作。最后，我们描述了将LAMP从台式机转换到现场的挑战，以及未来LAMP测定技术开发和性能报告的机会。

专家意见：尽管LAMP在学术研究界很流行，并且最近由于COVID-19大流行而对LAMP的兴趣激增，但在LAMP的基本理解上仍有许多领域需要改进，这需要提高LAMP的领域。 LAMP分析方法的开发和表征。