ABSTRACT

The purpose of this study was to investigate the optimum conditions of several factors (i.e. types and concentration of acetate, aeration rate, pH control) for maximizing the mixotrophic cultivation of Scenedesmus acuminatus using acetate as an organic carbon source. When acetate was used, dissolved oxygen (DO) was quickly consumed and resulted in an anoxic condition for 52 h. Then, DO increased quickly by photosynthetic reaction. Whenever we put acetate in a reactor after DO was recovered to higher than 7 mg/L, cells were quickly grown via cell respiration, which subsequently resulted in an anoxic condition. Compared to aeration, ammonium acetate, ammonium acetate with aeration tests, the highest maximum biomass productivity of 0.73 g/L/d was obtained for pH control test with ammonium acetate dosage. From this study, we found that DO was essential for the fast assimilation of acetate and depleted DO was quickly regenerated for pH control test. From this fact, we found that pH control test with ammonium acetate dosage was the best cultivation method for Scenedesmus acuminatus under mixotrophic condition. These findings could be a useful reference for maximizing the cultivation of S. acuminatus in industrial-scale applications.

摘要

本研究的目的是探讨几个因素（即醋酸盐的类型和浓度、通气率、pH 控制）以最大限度地提高刺栅藻混合营养的最佳条件。使用乙酸盐作为有机碳源。当使用醋酸盐时，溶解氧 (DO) 很快被消耗，并导致 52 小时的缺氧条件。然后，DO 通过光合反应迅速增加。每当我们在 DO 恢复到高于 7 mg/L 后将醋酸盐放入反应器中时，细胞通过细胞呼吸快速生长，随后导致缺氧状态。与曝气、醋酸铵、醋酸铵曝气试验相比，醋酸铵投加量pH控制试验获得最高的最大生物质生产率0.73 g/L/d。从这项研究中，我们发现 DO 对于乙酸盐的快速同化至关重要，并且耗尽的 DO 可以快速再生用于 pH 控制测试。从这个事实，我们发现用醋酸铵剂量控制pH值是最好的培养方法。混合营养条件下的尖刺栅藻。这些发现可以为在工业规模应用中最大限度地培养S. acuminatus提供有用的参考。