Two water soluble complexes with Co^II and Cu^II ions were synthesized using a novel β-cyclodextrin based azo-functionalized Schiff base as a ligand. The Schiff base and its metal complexes were characterized by different physico-chemical and spectroscopic methods. From the analyses of the experimental data, distorted octahedral geometry has been assigned for both the metal complexes. The binding interactions between the metal complexes and DNA were investigated by means of a thermal denaturation study and viscosity measurements as well as by electronic absorption and fluorescence spectroscopy. The DNA cleavage efficacy of the metal complexes was also studied by agarose gel electrophoresis using pBR DNA. These studies revealed that both the metal complexes followed an intercalative mode of binding to calf thymus (CT)-DNA and also effectively cleaved the supercoiled pBR DNA. The Co^II complex, however, more efficiently cleaved CT-DNA than the Cu^II complex as much as the experimental results are concerned.

两种与Co ^II和Cu ^II形成的水溶性络合物使用新型的基于β-环糊精的偶氮官能化席夫碱作为配体合成离子。Schiff碱及其金属配合物通过不同的物理化学和光谱方法表征。通过对实验数据的分析，已为两种金属配合物指定了扭曲的八面体几何形状。通过热变性研究和粘度测量以及电子吸收和荧光光谱法研究了金属络合物和DNA之间的结合相互作用。还通过使用pBR DNA的琼脂糖凝胶电泳研究了金属配合物的DNA切割功效。这些研究表明，两种金属配合物均遵循与小牛胸腺（CT）-DNA结合的插入模式，并且还有效切割了超螺旋pBR DNA。公司^II复杂，但是，更有效地裂解CT-DNA比的Cu ^II络合物作为作为实验结果而言得多。