Salivary cortisol concentration and its circadian variation were detected by optimizing the ionic concentration of the solution during the immobilization of aptamers on a field-effect transistor biosensor. This was achieved by modifying our previously developed technique of detecting uncharged cortisol using the transformation of negatively charged aptamers by controlling the aptamer spacing. This spacing control was achieved by synthesizing pseudo-cortisol-binding aptamers with a guanine-quadruplex during the immobilization of the aptamers in a highly concentrated solution of K⁺.

中文翻译：

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钾调节的皮质醇适体的场效应晶体管生物传感器的钾固定化，以检测适体转化的电荷分布变化

通过优化适体在场效应晶体管生物传感器上固定过程中溶液的离子浓度，可以检测唾液皮质醇浓度及其昼夜变化。这是通过修改我们以前开发的检测不带电皮质醇的技术来实现的，该技术通过控制适体间距来带负电适体的转化。这种间隔控制是通过将拟南芥与皮质醇结合的适体在鸟嘌呤-四链体固定在高浓度的K ⁺溶液中的过程中完成的。