BACKGROUND The non-grain crop cassava has attracted intense attention in the biorefinery process. However, efficient biorefinery of the whole-cassava is faced with some challenges due to the existence of strain inhibition and refractory cellulose during the citrate production process. RESULTS Here, a novel breeding method-atmospheric room temperature plasma (ARTP) was applied for the strain improvement of citrate-producing strain Aspergillus niger from the whole-cassava. The citrate yield of the mutant obtained by ARTP mutagenesis increased by 36.5% in comparison with the original strain. Moreover, CA fermentation was further improved on basis of the enhanced co-saccharification strategy by supplementing glucoamylase and cellulase. The fermentation efficiency increased by 35.8% with a 17.0 g/L reduction in residual sugar on the pilot scale. CONCLUSION All these results confirmed that combination of the novel breeding method and enhanced co-saccharification strategy could efficiently refine the whole-cassava. It also provides inspiration for the production of value-added products and waste disposal in the agro-based industries. This article is protected by copyright. All rights reserved.

中文翻译：

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使用ARTP突变的黑曲霉和增强的共糖化策略对全木薯进行高效生物精炼以生产柠檬酸盐

背景技术非粮食作物木薯在生物精炼过程中引起了强烈关注。然而，由于柠檬酸盐生产过程中存在菌株抑制和难降解纤维素，全木薯的高效生物精炼面临一些挑战。结果在此，采用常压室温等离子体（ARTP）新育种方法对全木薯产柠檬酸黑曲霉进行菌株改良。与原始菌株相比，通过ARTP诱变获得的突变体的柠檬酸产量增加了36.5%。此外，在增强型共糖化策略的基础上，通过补充葡糖淀粉酶和纤维素酶，进一步改进了CA发酵。发酵效率提高了 35.8%，中试规模的残糖减少了 17.0 g/L。结论所有这些结果都证实了新的育种方法和增强的共糖化策略相结合可以有效地提炼全木薯。它还为农业产业中增值产品的生产和废物处理提供了灵感。本文受版权保护。版权所有。