Surface functionalization of silica nanoparticles (SiNPs) with biomolecules, such as amino acids, amino sugars and carbohydrates was performed to produce surface-functionalized silica nanoparticles (f-SiNPs) through physical adsorption on the surface of colloidal SiNPs. The structural and physicochemical properties of the SiNPs and f-SiNPs were determined using SEM, FTIR, UV, DLS and NMR spectroscopy. The size of the functionalized-silica nanoparticles is found in the range of ~28.0 to 141.0 nm while the surface charge of the nanoparticles was observed to be negative except for f-SiNPs-Met, f-SiNPs-Glu and f-SiNPs-Gly. The SEM images of surface morphology of f-SiNPs described the differences from the SEM images of SiNPs that corroborated the silica nanoparticles’ functionalization. The synthesized SiNPs and f-SiNPs with biomolecules have been used for interaction with plant DNA isolated from the Bixa orellana L. plant. DNA interaction was quantified using agarose gel electrophoresis, visualized with a marker, and the purity was examined by the absorption ratio of A260: A280 (1.81) of isolated DNA and the quantity of the estimated dsDNA was found to be 1495 μg/mL. Interaction of DNA with alanine-SiNPs have shown greater intercalation properties due to its hydrophobic nature that protected the action of DNA molecules, whereas lysine-encapsulated nanoparticles have revealed DNA de-naturalization or replication.

Graphical abstract

中文翻译：

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二氧化硅纳米粒子和生物分子功能化的二氧化硅纳米粒子与Bixa orellana L.植物DNA的分子相互作用

使用生物分子（例如氨基酸，氨基糖和碳水化合物）对二氧化硅纳米粒子（SiNPs）进行表面功能化，以通过在胶体SiNPs表面上的物理吸附产生表面官能化的二氧化硅纳米粒子（f -SiNPs）。使用SEM，FTIR，UV，DLS和NMR光谱测定了SiNPs和f -SiNPs的结构和理化性质。发现官能化的二氧化硅纳米颗粒的尺寸在〜28.0至141.0nm的范围内，而观察到纳米颗粒的表面电荷为负，除了f -SiNPs-Met，f -SiNPs-Glu和f -SiNPs-Gly之外。 。f表面形貌的SEM图像-SiNPs描述了与证实二氧化硅纳米颗粒功能化的SiNPs的SEM图像的差异。具有生物分子的合成的SiNP和f- SiNP已用于与从Bixa orellana L.植物分离的植物DNA相互作用。使用琼脂糖凝胶电泳对DNA相互作用进行定量，并用标记物进行可视化，并通过分离的DNA的A260：A280（1.81）的吸收比检查纯度，发现估计的dsDNA的量为1495μg/ mL。DNA与丙氨酸-SiNPs的相互作用由于其疏水性保护了DNA分子的作用而显示出更大的嵌入性质，而赖氨酸包封的纳米颗粒则显示了DNA的变性或复制。

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