The main objective of our study was to evaluate the effectiveness of Trichoderma viride BHU-2953 as a single inoculant during seed-priming to enhance phosphorus (P) uptake in soybean. A pot experiment was conducted, taking six treatments, in a completely randomized block design to assess the P uptake, root length, apparent phosphorus recovery (APR), rhizospheric phosphatase activity, pH, soil-P status, dehydrogenase activity, and fungal colony-forming unit (CFU g⁻¹) in three different soybean growth stages through seed-priming with T. viride along with graded fertilizer P-doses. Significantly (P < 0.05) higher soil phosphatase activity, dehydrogenase activity, CFU of T. viride, and P-content in soybean vegetative parts were observed in bio-primed treatments as compared to control and RDF (full recommended dose of P-fertilizer without seed-priming), while seed-P (%), APR (%), and root lengths of RDF were found significantly (P < 0.05) lower than bio-primed soybeans with 90% RDF but were at par with 80% RDF. Higher positive correlations between CFU and acid phosphatase (R² = 0.89, 0.9, and 0.89; P < 0.05) and between CFU and alkaline phosphatase activities (R² = 0.98, 0.96, and 0.97; P < 0.05) at 30, 50, and 75 DAS indicate that T. viride mediated higher soil phosphatase activities. Higher P-recoveries of bio-primed soybeans, received 90% and 80% RDF, were achieved mainly due to soil applied-P solubilization through enhanced acid phosphatase activities along with better soil exploration by plant roots. Thus, seed-priming with T. viride BHU-2953 can reduce up to 20% of the recommended P-dose in soybeans.

我们研究的主要目的是评估在种子引发期间增加大豆中磷（P）吸收的木霉木霉BHU-2953作为单一接种剂的有效性。在完全随机的区组设计中进行了六种处理的盆栽试验，以评估P吸收，根长，表观磷回收率（APR），根际磷酸酶活性，pH，土壤P状态，脱氢酶活性和真菌菌落-在三个不同的大豆生长阶段中，通过用T. viride和分级肥料P剂量对底种子进行种子灌浆，形成单位（CFU g ^-1）。T. viride的 土壤磷酸酶活性，脱氢酶活性和CFU显着（P <0.05）与对照和RDF（建议全剂量的P肥料，无种子灌浆）相比，在生物灌浆处理中观察到大豆营养部位的P含量，而种子P（％），APR（％）和发现RDF的根长显着（P  <0.05）比具有90％RDF的生物引发大豆低，但与80％RDF相当。CFU与酸性磷酸酶之间的正相关性较高（R ²  = 0.89、0.9和0.89；P  <0.05），CFU与碱性磷酸酶活性之间的正相关性较高（R ²  = 0.98、0.96和0.97；P  <0.05）在30、50，和75 DAS表示T. viride介导的较高的土壤磷酸酶活性。生物引发大豆的P回收率较高，分别达到RDF的90％和80％，这主要归因于通过提高酸性磷酸酶的活性而土壤施用的P增溶作用以及植物根部对土壤的更好勘探。因此，用T. viride BHU-2953进行种子引发可减少大豆中推荐的P剂量的20％。