Tumor migration is the critical step that lead to the migration in retinoblastoma (RB), in which microRNAs (miRNAs) play important roles. This study aimed to investigate the role of microRNA-4319 (miR-4319) in the development of retinoblastoma by identifying its targets, as well as its underlying regulatory mechanisms. Our data shown that miR-4319 was downregulated in RB tissues and RB cell lines. Enhanced miR-4319 suppressed cell proliferation, migration, invasion and EMT progress, promoted cell apoptosis in SO-RB50 and RB-Y79 cells. Of note, extracellular matrix metalloproteinase inducer (EMMPRI/CD147) was identified as a direct target gene for miR-4319. MMPs were regulated by CD147 and participated in the miR-4319 regulatory network in SO-RB50 cells. In addition, overexpression of CD147 abrogated the inhibitory effect of miR-4319 on RB cells. In summary, miR-4319 overexpression suppressed cell proliferation, migration and invasion may through suppressing the CD147 mediated MMPs expression, suggesting that miR-4319 may serve as a potential diagnostic biomarker and treatment target for RB.

肿瘤迁移是导致视网膜母细胞瘤（RB）迁移的关键步骤，其中microRNA（miRNA）发挥着重要作用。本研究旨在通过确定 microRNA-4319 (miR-4319) 的靶标及其潜在的调控机制来研究 microRNA-4319 (miR-4319) 在视网膜母细胞瘤发展中的作用。我们的数据显示 miR-4319 在 RB 组织和 RB 细胞系中下调。增强的miR-4319抑制细胞增殖、迁移、侵袭和EMT进程，促进SO-RB50和RB-Y79细胞的细胞凋亡。值得注意的是，细胞外基质金属蛋白酶诱导物 (EMMPRI/CD147) 被确定为 miR-4319 的直接靶基因。MMP 受 CD147 调节，并参与 SO-RB50 细胞中的 miR-4319 调节网络。此外，CD147的过表达消除了miR-4319对RB细胞的抑制作用。