The goal of the present study was to characterize a novel bovine intestinal myofibroblast (BT-IMF) cell line isolated from a fetal bovine intestine. This cell type is of importance as intestinal myofibroblasts play a key role in controlling intestinal epithelial cell proliferation, intestinal regulation, wound healing, epithelial cell turnover, and structural support. The present work demonstrates that BT-IMF cells could be successfully cryopreserved and thawed and cultured past 25 passages. Immunocytochemical staining of the BT-IMF cell line was positive for vimentin and smooth muscle actin (α-SMA) and negative for pancytokeratin, suggesting that the cells are myofibroblastic in type. Growth kinetic experiments demonstrate that hydrocortisone negatively impacts BT-IMF growth and non-essential amino acids enhance its proliferation. Inosine monophosphate (IMP) is a dietary nucleotide and is essential for supporting animal health. Stimulation with IMP bound to a novel phytoglycogen-based nanocarrier (IMP-NP) showed enhanced cell proliferation. BT-IMF provides a new tool for studying bovine cells in vitro and may be of particular interest for cultured meat manufacturing in the future.

本研究的目的是表征从胎牛肠道分离的新型牛肠肌成纤维细胞 (BT-IMF) 细胞系。这种细胞类型很重要，因为肠肌成纤维细胞在控制肠上皮细胞增殖、肠道调节、伤口愈合、上皮细胞更新和结构支持方面起着关键作用。目前的工作表明 BT-IMF 细胞可以成功冷冻保存、解冻和培养超过 25 代。BT-IMF 细胞系的免疫细胞化学染色波形蛋白和平滑肌肌动蛋白 (α-SMA) 呈阳性，全细胞角蛋白呈阴性，表明细胞是肌纤维母细胞类型。生长动力学实验表明氢化可的松对 BT-IMF 生长产生负面影响，非必需氨基酸会增强其增殖。肌苷单磷酸 (IMP) 是一种膳食核苷酸，对支持动物健康至关重要。将 IMP 与一种新型的基于植物糖原的纳米载体 (IMP-NP) 结合进行刺激，显示出增强的细胞增殖。BT-IMF 为体外研究牛细胞提供了一种新工具，未来可能对培养肉制造特别感兴趣。