For mass culture of photosynthetic green microalgae, industrial flue gases can represent a low-cost resource of CO₂. However, flue gases are often avoided, because they often also contain high levels of SO₂ and/or NO₂, which cause significant acidification of media to below pH 3 due to production of sulfuric and nitric acid. This creates an unsuitable environment for the neutrophilic microalgae commonly used in large-scale commercial production. To address this issue, we have looked at selecting acid-tolerant microalgae via growth at pH 2.5 carried out with samples bioprospected from an active smelter site. Of the eight wild samples collected, one consisting mainly of Coccomyxa sp. grew at pH 2.5 and achieved a density of 640 mg L⁻¹. Furthermore, three previously bioprospected green microalgae from acidic waters (pH 3–4.5) near abandoned mine sites were also re-acclimated down to their in-situ pH environment after approximately 4 years spent at neutral pH. Of those three, an axenic culture of Coccomyxa sp. was the most successful at re-acclimating and achieved the highest density of 293.1 mg L⁻¹ and maximum daily productivity of 38.8 mg L⁻¹ day⁻¹ at pH 3. Re-acclimation of acid-tolerant species is, therefore, achievable when directly placed at their original pH, but gradual reduction in pH is recommended to give the cells time to acclimate.

对于光合作用绿色微藻的大规模培养，工业烟气可以代表低成本的CO ₂资源。但是，通常避免使用烟道气，因为它们通常还含有高含量的SO ₂和/或NO ₂，由于产生硫酸和硝酸，会导致介质的显着酸化至pH 3以下。这为大规模商业生产中常用的嗜中性微藻创造了不合适的环境。为了解决这个问题，我们已经研究了如何通过在pH 2.5上生长来选择耐酸微藻，其中从活动的冶炼厂进行了生物勘探。在收集的八个野生样品中，一个主要由球孢菌属组成。在pH 2.5下生长并达到640 mg L的密度^-1。此外，在中性pH值下使用约4年后，在废弃矿区附近的酸性水（pH 3-4.5）中，有3种先前被生物勘探的绿色微藻也重新适应了原位pH环境。在这三者中，球孢菌属的无性培养物。是最成功的重新适应环境，并且在pH 3时达到最高密度293.1 mg L ^-1和最大日生产力38.8 mg L ^-1  day ^-1。因此，在以下情况下可以实现耐酸物质的重新适应直接将其置于原始pH值下，但建议逐渐降低pH值，以使细胞有时间适应环境。