Plant chemistry plays an important role in mediating resistance against insect herbivores. Depending on insect species and feeding strategies, plant defenses can prevent insect attacks, impact digestive physiology and influence reproductive output. The Asian longhorned beetle (Anoplophora glabripennis) is an invasive, tree-killing insect that exploits multiple host plant species. Anoplophora glabripennis is documented to attack Populus spp., but the ability for adults to consume Populus varies between beetle populations. In this study, we explored the influence of salicinoid phenolic glycosides, known to be potent chemical defenses in Populus against generalist insects, on A. glabripennis adult behavior and fecundity. We performed a series of choice- and no-choice experiments using extracts of salicinoids applied to plants that do not produce these compounds. Salicinoids affected A. glabripennis performance in a dose-dependent manner, with higher concentrations significantly reducing feeding. Egg development was reduced by > 90% in females fed salicinoids at ~ 1.3% dry weight. We then compared salicinoids present in feeding material vs. frass and found both a decrease in concentration and a shift in composition of these compounds. However, some of the more complex salicinoids were unchanged in the excrement, suggesting minimal detoxification had occurred. Our results show that salicinoids disrupt egg development in A. glabripennis, thereby reducing fitness. These results have implications for how A. glabripennis metabolizes plant defense chemicals and how tolerance may vary among populations.

植物化学在介导对食草动物的抗性中起重要作用。根据昆虫种类和摄食策略，植物防御可以防止昆虫侵袭，影响消化生理并影响生殖产量。亚洲长角甲虫（Anoplophora glabripennis）是一种入侵性，杀死树木的昆虫，利用多种寄主植物物种。已有记载，无节金虫攻击胡杨属，但成虫食用胡杨的能力因虫种群而异。在这项研究中，我们探讨了水杨素酚类苷对已知的对普通昆虫的有效化学防御作用，该化合物在杨树中对普通昆虫具有有效的防御作用。成人的行为和生殖力。我们将水杨素提取物应用于不产生这些化合物的植物，进行了一系列选择和不选择的实验。Salicinoids影响A.天牛以剂量依赖方式表现，具有较高浓度显著减少馈送。饲喂水杨素的雌性干重约1.3％时，卵的发育减少> 90％。然后，我们比较了饲料原料和苦味素中存在的类水杨素，发现这些化合物的浓度降低且组成发生变化。但是，一些更复杂的水杨素在排泄物中未发生变化，这表明已发生了最少的排毒。我们的研究结果表明，类水杨素会破坏A. glabripennis的卵发育，从而降低健身度。这些结果暗示着农杆菌如何代谢植物防御化学物质以及耐受性如何在种群之间变化。