Photoexcited molecular probes for selective and revertible imaging of cellular reactive oxygen species,Organic Chemistry Frontiers

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Photoexcited molecular probes for selective and revertible imaging of cellular reactive oxygen species
Organic Chemistry Frontiers ( IF 4.6 ) Pub Date : 2021-1-20 , DOI: 10.1039/d0qo01260b
Ying Wen _{1,

2,

3,

4} , Zhiqing Long _{1,

2,

3,

4} , Fangjun Huo _{2,

3,

4,

5} , Caixia Yin _{1,

2,

3,

4}

Affiliation

Redox homeostasis is key to maintaining the normal physiological status of living cells. The redox state of cells is primarily a consequence of the precise balance between levels of reactive oxygen species (ROS) and biological reductants. Optical imaging has become one of the most preferred methods for biological sensing in complicated systems, due to its easy operation, good selectivity, and superior sensitivity. The development of optical tools to trace and label ROS is a formidable and challenging task due to the high reactivity, short lifetimes, and extremely low concentrations of ROS. In recent advances, probes that serve as ‘modulators’ for labeling fluctuation change of ROS levels, rather than imaging the transient ROS bursts, have been widely reported. Classic examples of probes for selectively and revertibly imaging cellular ROS, including fluorescent, phosphorescent, and photoacoustic probes, are summarized and several possible future directions in this field are discussed in this review. The advanced probes can provide a powerful tool to study redox homeostasis between a specific ROS and biological reductants, and may establish foundations for biomedical research on dynamic real-time monitoring of ROS in living systems.

中文翻译：

光激发分子探针用于细胞活性氧的选择性和可逆成像

氧化还原稳态是维持活细胞正常生理状态的关键。细胞的氧化还原状态主要是活性氧（ROS）和生物还原剂之间精确平衡的结果。光学成像由于其易于操作，良好的选择性和优越的灵敏度，已成为复杂系统中生物传感的最优选方法之一。由于高反应活性，短寿命和极低的ROS浓度，开发用于跟踪和标记ROS的光学工具是一项艰巨而具有挑战性的任务。在最近的进展中，已广泛报道了用作“调节剂”以标记ROS水平波动变化而不是对瞬时ROS突发成像的探针。用于选择性和可逆地对细胞ROS成像的探针的经典示例，本文综述了包括荧光，磷光和光声探针在内的各种探针，并对该领域中一些可能的未来方向进行了讨论。先进的探针可以为研究特定ROS与生物还原剂之间的氧化还原稳态提供强大的工具，并且可以为生物医学研究中对生命系统中ROS的动态实时监测奠定基础。

更新日期：2021-01-20

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