Pb(II) detection and versatile bio-imaging of green-emitting carbon dots with excellent stability and bright fluorescence,Nanoscale

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Pb(II) detection and versatile bio-imaging of green-emitting carbon dots with excellent stability and bright fluorescence
Nanoscale ( IF 5.8 ) Pub Date : 2021-1-2 , DOI: 10.1039/d0nr07245a
Zishan Sun _{1,

2,

3,

4,

5} , Yuping Zhou _{3,

4,

5,

6,

7} , Weiying Zhou _{1,

2,

3,

4,

5} , Jiabao Luo _{1,

2,

3,

4,

5} , Ruiyuan Liu _{3,

4,

5,

6,

7} , Xinguo Zhang _{1,

2,

3,

4,

5} , Liya Zhou _{5,

8,

9,

10} , Qi Pang _{5,

8,

9,

10}

Affiliation

Green-emitting carbon dots (G-CDs) were synthesized via a simple and green hydrothermal method using betaine hydrochloride and sulfadiazine as carbon and nitrogen sources, respectively. Excellent luminescence stability with varying pH, salt concentrations, temperature is found with excitation-independent emission. G-CDs can be successfully used for the detection of Pb(II) in the range of 0–200 μM. There was good linear relationship between the Pb(II) concentration and G-CD fluorescence intensity with a correlation coefficient of 0.993, and the limit of detection (LOD) was 3.0174 μmol L⁻¹. Due to its good biocompatibility, G-CDs can be successfully applied to zebrafish imaging as well as cell imaging, and the results show that G-CDs is more suitable for the zebrafish embryo imaging. Our results suggested that the obtained G-CDs can be used as multifunctional probes, highlighting their potential in different biological studies.

中文翻译：

具有出色稳定性和明亮荧光的绿色发光碳点的Pb（II）检测和多功能生物成像

通过简单且绿色的水热方法，分别使用甜菜碱盐酸盐和磺胺嘧啶作为碳源和氮源，合成了绿色发光碳点（G-CD）。在不依赖激发的情况下，发现在不同的pH，盐浓度，温度下具有出色的发光稳定性。G-CD可以成功地用于检测0–200μM范围内的Pb（II）。Pb（II）浓度与G-CD荧光强度之间存在良好的线性关系，相关系数为0.993，检出限（LOD）为3.0174μmolL ^-1。由于其良好的生物相容性，G-CDs可成功应用于斑马鱼成像以及细胞成像，结果表明G-CDs更适合于斑马鱼胚胎成像。我们的结果表明，获得的G-CDs可用作多功能探针，突出了它们在不同生物学研究中的潜力。

更新日期：2021-01-20

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