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Pb(II) detection and versatile bio-imaging of green-emitting carbon dots with excellent stability and bright fluorescence
Nanoscale ( IF 5.8 ) Pub Date : 2021-1-2 , DOI: 10.1039/d0nr07245a
Zishan Sun 1, 2, 3, 4, 5 , Yuping Zhou 3, 4, 5, 6, 7 , Weiying Zhou 1, 2, 3, 4, 5 , Jiabao Luo 1, 2, 3, 4, 5 , Ruiyuan Liu 3, 4, 5, 6, 7 , Xinguo Zhang 1, 2, 3, 4, 5 , Liya Zhou 5, 8, 9, 10 , Qi Pang 5, 8, 9, 10
Affiliation  

Green-emitting carbon dots (G-CDs) were synthesized via a simple and green hydrothermal method using betaine hydrochloride and sulfadiazine as carbon and nitrogen sources, respectively. Excellent luminescence stability with varying pH, salt concentrations, temperature is found with excitation-independent emission. G-CDs can be successfully used for the detection of Pb(II) in the range of 0–200 μM. There was good linear relationship between the Pb(II) concentration and G-CD fluorescence intensity with a correlation coefficient of 0.993, and the limit of detection (LOD) was 3.0174 μmol L−1. Due to its good biocompatibility, G-CDs can be successfully applied to zebrafish imaging as well as cell imaging, and the results show that G-CDs is more suitable for the zebrafish embryo imaging. Our results suggested that the obtained G-CDs can be used as multifunctional probes, highlighting their potential in different biological studies.

中文翻译:

具有出色稳定性和明亮荧光的绿色发光碳点的Pb(II)检测和多功能生物成像

通过简单且绿色的水热方法,分别使用甜菜碱盐酸盐和磺胺嘧啶作为碳源和氮源,合成了绿色发光碳点(G-CD)。在不依赖激发的情况下,发现在不同的pH,盐浓度,温度下具有出色的发光稳定性。G-CD可以成功地用于检测0–200μM范围内的Pb(II)。Pb(II)浓度与G-CD荧光强度之间存在良好的线性关系,相关系数为0.993,检出限(LOD)为3.0174μmolL -1。由于其良好的生物相容性,G-CDs可成功应用于斑马鱼成像以及细胞成像,结果表明G-CDs更适合于斑马鱼胚胎成像。我们的结果表明,获得的G-CDs可用作多功能探针,突出了它们在不同生物学研究中的潜力。
更新日期:2021-01-20
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