Abstract: Neutrophils are the most abundant immune cell type in the blood and constitute the first line of defense against invading pathogens. Despite their important role in many diseases, they are challenging to study due to their short life span and the inability to cryopreserve or expand them in vitro. Thus, research into neutrophils has to rely on cells freshly isolated from peripheral blood of human donors, introducing donor-dependent variation in the experimental data. To counteract these problems, researchers tried to develop adequate cell models, such as cell lines. For those functional studies that cannot rely on cell models, a standardization of protocols regarding neutrophil purification and culturing could be a solution. In this review, we provide an overview of the most commonly used models for neutrophil function (HL-60, PLB-985, NB4, Kasumi-1 and induced pluripotent stem cells). In addition, we describe the effects of glucose concentration, pH, oxygen tension and temperature on neutrophil function.

Keywords: neutrophils, HL-60, PLB-985, NB4, Kasumi-1, induced pluripotent stem cells


中文翻译：

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体外研究中性粒细胞功能：细胞模型和环境因素

摘要：中性粒细胞是血液中最丰富的免疫细胞类型，是抵御入侵病原体的第一道防线。尽管它们在许多疾病中起着重要的作用，但由于它们的寿命短以及无法在体外冷冻保存或扩增它们，因此仍面临研究挑战。因此，对中性粒细胞的研究必须依赖于从人类供体外周血中新鲜分离的细胞，从而在实验数据中引入了供体依赖性变异。为了解决这些问题，研究人员试图开发适当的细胞模型，例如细胞系。对于那些不能依赖细胞模型的功能性研究，可以将中性粒细胞纯化和培养的协议标准化。在本文中，我们概述了嗜中性粒细胞功能最常用的模型（HL-60，PLB-985，NB4，Kasumi-1和诱导性多能干细胞）。此外，我们描述了葡萄糖浓度，pH，氧张力和温度对嗜中性粒细胞功能的影响。

关键词：中性粒细胞HL-60 PLB-985 NB4 Kasumi-1诱导多能干细胞