Surface engineered amphiphilic carbon dots: solvatochromic behavior and applicability as a molecular probe,Journal of Materials Chemistry B

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Surface engineered amphiphilic carbon dots: solvatochromic behavior and applicability as a molecular probe
Journal of Materials Chemistry B ( IF 6.1 ) Pub Date : 2020-12-30 , DOI: 10.1039/d0tb02007a
Subrata Pandit _{1,

2,

3,

4} , Sudipta Mondal _{1,

2,

3,

4} , Mrinmoy De _{1,

2,

3,

4}

Affiliation

Carbon dots (C-dots) have attracted great attention in the fields of nanotechnology and bioengineering owing to their unique and tunable optical properties with excellent photoluminescence characteristics. Herein, we have engineered amphiphilic C-dots (AC-dots) using positional isomers of diamino benzene with citric acid under mild microwave irradiation to minimize any background reactions. The optical properties changed from excitation-dependent to excitation-independent depending on the isomer used. This unique optical property of the AC-dots was studied in the presence of various solvents and we extensively inspected the AC-dot–solvent interactions. The intensity of the emission wavelength varied with solvent polarity and showed a linear relationship. Furthermore, we extended this property to investigate the molecular environment in biomolecular systems such as proteins. Interestingly, we found that, in the presence of various proteins, the emission intensity was enhanced, quenched or remained unchanged depending on the nature of the protein surface. The mode of interaction between AC-dots and protein was determined using temperature-dependent fluorescence spectroscopy. This study could provide vital information about the surfaces of proteins and the potential application of C-Dots as a fluorescent probe to detect biological molecules and environments.

中文翻译：

表面工程两亲性碳点：溶剂变色行为和作为分子探针的适用性

碳点（C-dots）由于其独特且可调节的光学特性以及出色的光致发光特性而在纳米技术和生物工程领域引起了极大的关注。本文中，我们在温和的微波辐射下使用二氨基苯与柠檬酸的位置异构体设计了两亲性C点（AC点）。取决于所使用的异构体，光学性质从依赖于激发变为不依赖于激发。在各种溶剂的存在下研究了AC点的这种独特的光学性质，我们广泛检查了AC点与溶剂的相互作用。发射波长的强度随溶剂极性而变化，并呈线性关系。此外，我们扩展了此属性，以研究生物分子系统（例如蛋白质）中的分子环境。有趣的是，我们发现，在各种蛋白质的存在下，发射强度会根据蛋白质表面的性质而增强，淬灭或保持不变。AC点和蛋白质之间的相互作用模式使用温度依赖性荧光光谱法确定。这项研究可以提供有关蛋白质表面的重要信息以及C-Dots作为荧光探针检测生物分子和环境的潜在应用。AC点和蛋白质之间的相互作用模式使用温度依赖性荧光光谱法确定。这项研究可以提供有关蛋白质表面的重要信息以及C-Dots作为荧光探针检测生物分子和环境的潜在应用。AC点和蛋白质之间的相互作用模式使用温度依赖性荧光光谱法确定。这项研究可以提供有关蛋白质表面的重要信息以及C-Dots作为荧光探针检测生物分子和环境的潜在应用。

更新日期：2021-01-20

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