High-throughput experimentation has become increasingly important in the identification and optimization of enzymatic reactions. Unfortunately, traditional methods of preparing biocatalyst screens can be operationally demanding and expose the catalyst to potentially damaging conditions. In an advance on traditional methods, we were able to noncovalently load enzyme-containing powders on inert carrier beads, which, in concert with solid-dispensing robots, enable accurate submilligram dosing of catalyst to microtiter screening reactions. This formulation has been demonstrated to possess comparable activity and selectivity to traditional catalyst dispersal methods with the bonus of increased stability. This technique will dramatically increase the ease, speed, and accuracy with which enzymatic reactions can be screened.

中文翻译：

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固体，非共价配比的生物催化剂，可快速，精确地在微量滴定板上投药

高通量实验在酶促反应的鉴定和优化中变得越来越重要。不幸的是，制备生物催化剂筛的传统方法可能在操作上要求很高，并使催化剂暴露于潜在的破坏性条件下。在传统方法的一项进步中，我们能够将含酶的粉末非共价地负载在惰性载体微珠上，与固体分装机器人配合使用，可以精确地将亚毫克剂量的催化剂用于微量滴定筛选反应。已证明该配方具有与传统催化剂分散方法相当的活性和选择性，并具有增加的稳定性。此技术将大大提高筛选酶促反应的简便性，速度和准确性。