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Single Cell Analysis of Bistable Expression of Pathogenicity Island 1 and the Flagellar Regulon in Salmonella enterica
Microorganisms ( IF 4.1 ) Pub Date : 2021-01-20 , DOI: 10.3390/microorganisms9020210
María Antonia Sánchez-Romero , Josep Casadesús

Bistable expression of the Salmonella enterica pathogenicity island 1 (SPI-1) and the flagellar network (Flag) has been described previously. In this study, simultaneous monitoring of OFF and ON states in SPI-1 and in the flagellar regulon reveals independent switching, with concomitant formation of four subpopulations: SPI-1OFF FlagOFF, SPI-1OFF FlagON, SPI-1ON FlagOFF, and SPI-1ON FlagON. Invasion assays upon cell sorting show that none of the four subpopulations is highly invasive, thus raising the possibility that FlagOFF cells might contribute to optimal invasion as previously proposed for SPI-1OFF cells. Time lapse microscopy observation indicates that expression of the flagellar regulon contributes to the growth impairment previously described in SPI-1ON cells. As a consequence, growth resumption in SPI-1ON FlagON cells requires switching to both SPI-1OFF and FlagOFF states.

中文翻译:

肠道沙门氏菌中致病岛1和鞭毛调节基因双稳态表达的单细胞分析

先前已经描述了肠沙门氏菌致病岛1(SPI-1)和鞭毛网络(Flag)的双稳态表达。在这项研究中,同时监视SPI-1和鞭毛调节器中的OFF和ON状态揭示了独立​​的切换,并伴随着四个亚群的形成:SPI-1 OFF标志OFF,SPI-1 OFF标志ON,SPI-1 ON标志OFF,并且SPI-1 ON标志为ON。细胞分选后的侵袭分析表明,这四个亚群均没有高度侵袭性,因此增加了将标志关闭的可能性如先前针对SPI-1 OFF细胞所提出的,细胞可能有助于最佳侵袭。延时显微镜观察表明,鞭毛调节子的表达有助于先前在SPI-1 ON细胞中描述的生长障碍。结果,在SPI-1 ON Flag ON单元中恢复生长需要同时切换到SPI-1 OFF和Flag OFF状态。
更新日期:2021-01-20
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