Potato (Solanum tuberosum L.) is the primary vegetable crop consumed worldwide and is largely affected by bacterial pathogens that can cause soft rot and blackleg disease. Recently, resistance to these diseases has been identified in the wild potato S. chacoense, and the mechanism of resistance is unknown. Here, it was hypothesized that S. chacoense stems or tubers have unique chemistry that confers resistance to the pathogen Pectobacterium brasiliense through bactericidal, bacteriostatic, or antivirulence activity. Stem and tuber metabolite extracts were collected from S. chacoense and tested for effects on Pectobacterium bacterial multiplication rates, and activity and expression of known exoenzymes and virulence genes using S. tuberosum extracts as a comparative control. Comparatively, the S. chacoense extracts did not affect bacterial multiplication rate; however, they did reduce pectinase, cellulase, and protease activities. The chemical extracts were profiled using a bioassay-guided fractionation, and a nontargeted metabolomics comparison of S. chacoense and S. tuberosum stems and tubers was performed. The data showed that selected alkaloids, phenolic amines, phenols, amines, and peptides are integrative chemical sources of resistance against the bacteria.

Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

马铃薯（Solaum tuberosum L.）是世界范围内消费的主要蔬菜作物，并且很大程度上受到细菌病原体的影响，这些病原体会引起软腐和黑腿病。最近，在野生马铃薯沙门氏菌中已经鉴定出对这些疾病的抗性，并且抗性的机理尚不清楚。在这里，有人推测S. chacoense茎或块茎具有独特的化学性质，为病原体的抗性黑腐brasiliense通过杀菌，抑菌，或antivirulence活动。从沙门氏菌中提取茎和块茎代谢物提取物，并测试其对果胶杆菌的影响马铃薯提取物作为对照，细菌繁殖率，已知外切酶和毒力基因的活性和表达。相比之下，瑞士链球菌提取物不影响细菌繁殖率。但是，它们确实降低了果胶酶，纤维素酶和蛋白酶的活性。使用生物测定指导的分级分析对化学提取物进行谱分析，并对沙棘链球菌和马铃薯块茎和块茎进行非目标代谢组学比较。数据表明，选定的生物碱，酚胺，酚，胺和肽是抵抗细菌的综合化学来源。

版权所有©2021作者。这是根据CC BY-NC-ND 4.0 International许可分发的开放获取文章。