ABSTRACT

Background: The factor behind the activation of the remnant odontogenic tissues and development of odontogenic cysts and tumors is poorly understood.This study aimed to investigate the presence of human cytomegalovirus (HCMV) in dentigerous cyst (DC), odontogenic keratocyst (OKC), and ameloblastoma (AB).

Methods: The study included 41 samples, which distributed into DC (n=13), OKC (n=12), and AB (n=16). Conventional PCR assay and IHC analysis were used to detect the HCMV-DNA and HCMV glycoprotein B (HCMV-gB) respectively.

Results: HCMV-DNA was detected in 10 samples (62.5%) of AB, four samples (30.8%) of DC, and three samples (25 %) of OKC respectively (χ² test = 1.195, p= 0.247). Meanwhile, HCMV-gB was found in 12 (75%) of AB, in 2 (15.4%) of DC, and absent in OKC (0.0%) (χ² test = 4.122, p= 0.042).

Conclusions: The high prevalence of HCMV inside the odontogenic epithelium of AB could indicate a possible role of the virus in the oncogenesis and/or oncomodulation of the AB. Additionally, we recommend the IHC for the detection of HCMV in the odontogenic tumors like AB.

摘要

背景：对残留牙源性组织激活和牙源性囊肿和肿瘤发展背后的因素知之甚少。本研究旨在调查人巨细胞病毒 (HCMV) 在牙源性囊肿 (DC)、牙源性角化囊 (OKC) 和牙源性角化囊肿 (OKC) 中的存在。成釉细胞瘤（AB）。

方法：本研究共纳入41个样本，分布于DC（n=13）、OKC（n=12）和AB（n=16）。分别使用常规PCR测定和IHC分析检测HCMV-DNA和HCMV糖蛋白B（HCMV-gB）。

结果：分别在AB的10个样本（62.5%）、DC的4个样本（30.8%）和OKC的三个样本（25%）中检测到HCMV-DNA（χ ²检验= 1.195，p= 0.247）。同时，在 12 (75%) AB、2 (15.4%) DC 中发现 HCMV-gB，在 OKC (0.0%) 中不存在 (χ ²检验 = 4.122，p = 0.042)。

结论： AB 牙源性上皮内 HCMV 的高流行率可能表明病毒在 AB 的肿瘤发生和/或肿瘤调节中可能发挥作用。此外，我们推荐使用 IHC 检测牙源性肿瘤（如 AB）中的 HCMV。