This highlight review overviews recent progress in fluorescence imaging technology for the alkaline-earth metal ions calcium, and magnesium, by small molecule-based fluorescent probes. Fluorescent probes for Ca²⁺ using 1,2-bis-(o-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid (BAPTA) as a recognition unit are introduced. Focus is set on the fluorescence characteristics and intracellular dynamics of Ca²⁺ fluorescent probes depending on the differences in fluorophore units. In addition, this review comprehensively covers Mg²⁺ fluorescent probes from Mg²⁺ selective ligands and fluorophore units to their application. In particular, the influence of the molecular structure of the fluorescent probes on organelle localization and fluorescence wavelength for imaging intracellular signals in organelles and intercellular multiple signal interactions were summarized, providing a strategy for probe design for chemists, and for selecting the appropriate imaging technique for biologists.

中文翻译：

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基于小分子的碱土金属离子荧光探针，用于细胞内和细胞间多信号成像

这篇综述着重概述了基于小分子的荧光探针对碱土金属离子钙和镁的荧光成像技术的最新进展。介绍了以1,2-双-（邻氨基苯氧基）乙烷-N，N，N'，N'-四乙酸（BAPTA）为识别单元的Ca ²⁺荧光探针。Ca ²⁺荧光探针的荧光特性和细胞内动力学取决于荧光团单位的差异。此外，这条全面涵盖的Mg ²⁺从镁荧光探针²⁺选择性配体和荧光团单元的应用。尤其是，总结了荧光探针的分子结构对细胞器定位和荧光波长的影响，以成像细胞器中的细胞内信号以及细胞间多信号相互作用，为化学家设计探针策略，并为选择合适的成像技术提供了策略。生物学家。