MYB106 is a negative regulator and a substrate for CRL3BPM E3 ligase in regulating flowering time in Arabidopsis thaliana,Journal of Integrative Plant Biology

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MYB106 is a negative regulator and a substrate for CRL3BPM E3 ligase in regulating flowering time in Arabidopsis thaliana
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2021-01-20 , DOI: 10.1111/jipb.13071
Liu Hong ₁ , Fangfang Niu ₁ , Youshun Lin ₁ , Shuang Wang _{1,

2} , Liyuan Chen _{1,

3} , Liwen Jiang _{1,

4}

Affiliation

Flowering time is crucial for successful reproduction in plants, the onset and progression of which are strictly controlled. However, flowering time is a complex and environmentally responsive history trait and the underlying mechanisms still need to be fully characterized. Post-translational regulation of the activities of transcription factors (TFs) is a dynamic and essential mechanism for plant growth and development. CRL3^BPM E3 ligase is a CULLIN3-based E3 ligase involved in orchestrating protein stability via the ubiquitin proteasome pathway. Our study shows that the mutation of MYB106 induced early flowering phenotype while over-expression of MYB106 delayed Arabidopsis flowering. Transcriptome analysis of myb106 mutants reveals 257 differentially expressed genes between wild type and myb106-1 mutants, including Flowering Locus T (FT) which is related to flowering time. Moreover, in vitro electrophoretic mobility shift assays (EMSA), in vivo chromatin immunoprecipitation quantitative polymerase chain reaction (ChIP-qPCR) assays and dual luciferase assays demonstrate that MYB106 directly binds to the promoter of FT to suppress its expression. Furthermore, we confirm that MYB106 interacts with BPM proteins which are further identified by CRL3^BPM E3 ligases as the substrate. Taken together, we have identified MYB106 as a negative regulator in the control of flowering time and a new substrate for CRL3^BPM E3 ligases in Arabidopsis.

中文翻译：

MYB106 是 CRL3BPM E3 连接酶的负调节剂和底物，用于调节拟南芥开花时间

开花时间对于植物的成功繁殖至关重要，植物的发生和进展受到严格控制。然而，开花时间是一个复杂且对环境敏感的历史特征，其潜在机制仍需充分表征。转录因子 (TF) 活性的翻译后调节是植物生长和发育的动态且必不可少的机制。CRL3 ^BPM E3 连接酶是一种基于 CULLIN3 的 E3 连接酶，通过泛素蛋白酶体途径参与协调蛋白质稳定性。我们的研究表明MYB106的突变诱导了早期开花表型，而MYB106 的过度表达延迟了拟南芥的开花。myb106的转录组分析突变体揭示了野生型和myb106 -1 突变体之间的 257 个差异表达基因，包括与开花时间相关的开花位点T ( FT )。此外，体外电泳迁移率变化测定 (EMSA)、体内染色质免疫沉淀定量聚合酶链反应 (ChIP-qPCR) 测定和双荧光素酶测定表明 MYB106 直接与FT的启动子结合以抑制其表达。此外，我们确认 MYB106 与 BPM 蛋白相互作用，后者由 CRL3 ^BPM进一步鉴定E3 连接酶作为底物。总之，我们已将 MYB106 鉴定为控制开花时间的负调节因子和拟南芥中CRL3 ^BPM E3 连接酶的新底物。

更新日期：2021-01-20

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