Mixed Surfactant‐Based Reverse Micellar Extraction Studies of Bovine Lactoperoxidase,Journal of Surfactants and Detergents

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Mixed Surfactant‐Based Reverse Micellar Extraction Studies of Bovine Lactoperoxidase
Journal of Surfactants and Detergents ( IF 1.6 ) Pub Date : 2021-01-19 , DOI: 10.1002/jsde.12489
Shwetha Karanth ₁ , Regupathi Iyyaswami ₁

Affiliation

The suitability of reverse micellar extraction for recovery of bovine lactoperoxidase (LP) from aqueous solution was evaluated using systems formed by ionic and nonionic surfactant mixtures. The influence of ionic surfactant concentration, organic solvent, and pH on the extraction of LP into the reverse micellar phase was studied. The Tween® series surfactants with Aerosol‐OT (bis‐(2‐ethylhexyl) sulfosuccinate) showed better extraction of LP in the reverse micelles (RM) compared to the Triton® and Span® series of surfactants. Complete extraction of LP from an aqueous phase of initial concentration 25 mg L⁻¹ occurred with the RM formed by 90 mM Aerosol‐OT/8 mM Tween® 80 in isooctane. The optimal pH, ionic strength, and positively charged ionic surfactant concentration for back extraction were also studied and a maximum of 95.5% back extraction efficiency and 66% LP activity recovery was obtained for a pH of 10.5,1 M KCl and 60 mM cetyltrimethylammonium bromide system.

中文翻译：

基于混合表面活性剂的牛乳过氧化物酶反胶束提取研究

使用由离子和非离子表面活性剂混合物形成的系统，评估了反胶束萃取从水溶液中回收牛乳过氧化物酶（LP）的适用性。研究了离子型表面活性剂浓度，有机溶剂和pH值对LP提取到胶束反相中的影响。与Triton®和Span®系列表面活性剂相比，含Aerosol-OT（双（2-乙基己基）磺基琥珀酸酯）的Tween®系列表面活性剂在反向胶束（RM）中显示出更好的LP提取。从初始浓度为25 mg L ^-1的水相中完全提取LP发生的原因是在异辛烷中由90 mM Aerosol-OT / 8 mMTween®80形成的RM。还研究了反萃取的最佳pH值，离子强度和带正电的离子表面活性剂浓度，当pH为10.5、1 M KCl和十六烷基十六烷基三甲基溴化铵时，最大反萃取效率和66％LP活性回收率。系统。

更新日期：2021-03-10

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