Necrotic enteritis and coccidiosis are the most economically detrimental enteric diseases of broiler chickens. This study aimed to investigate the association of DNA load of Clostridium perfringens, netB, and five Eimeria species (E. brunetti, E. maxima, E. necatrix, E. acervulina and E. tenella) in poultry house dust and pooled excreta with flock productive performance. The dust and pooled excreta from the floor were collected weekly at days 7, 14, 21, 28 and 35 of chicken age from 16 flocks of eight farms from two Australian integrator companies. The farms were ranked as high or low performers by each integrator according to the production performance of studied flocks. Eimeria tenella and necatrix were not detected in any farm while E. brunetti was detected in a low-performance farm and netB was detected in a high-performance farm. C. perfringens, E. acervulina and E. maxima DNA were detected on all farms with no significant differences in DNA load between high and low-performance farms or companies. The lack of association of pathogen DNA load and farm performance is possibly due to overall low to moderate pathogen DNA load detected in this study. Further studies on a larger number of farms are needed to determine whether these population level measurements of key pathogens based on PCR detection of nucleic acids are correlated with performance variables.

坏死性肠炎和球虫病是肉鸡经济上危害最大的肠道疾病。本研究旨在调查产气荚膜梭菌、netB和五种艾美球虫（E. brunetti、E. maxima、E . necatrix、E. acervulina和E. tenella）的 DNA 载量与家禽舍内灰尘和聚集排泄物的关联。生产性能。在鸡龄第 7、14、21、28 和 35 天，每周收集来自两家澳大利亚集成商公司的 8 个农场的 16 群鸡群的灰尘和地板上的汇集排泄物。每个集成商根据所研究鸡群的生产性能将农场分为高绩效或低绩效。柔嫩艾美耳球虫和球虫而任何场中没有检测到E. BRUNETTI是低性能场中检测和NETB高性能场中进行检测。在所有农场都检测到产气荚膜梭菌、E. acervulina和E. maxima DNA，高性能和低性能农场或公司之间的 DNA 负载没有显着差异。病原体 DNA 负载量与农场性能之间缺乏关联可能是由于本研究中检测到的总体低至中等病原体 DNA 负载量所致。需要对更多农场进行进一步研究，以确定这些基于核酸 PCR 检测的关键病原体种群水平测量值是否与性能变量相关。