Companion animals carry different microorganism of severely public health hazard for human; the kindness relation and contact between humans and companion animals may the route in the transmission of most zoonotic bacteria, including Methicillin-Resistant Staphylococcus aureus (MRSA). Therefore, the current study investigate the companion animals mainly dogs and cat as a reservoir for MRSA and the genetic similarity between the recovered strains of MRSA from such companion animals and their owners. One hundred swabs were collected under aseptic condition from companion animals and seventy swabs were collected from nasal and soft tissue of the infected owners in contact. All samples were examined with standard microbiological techniques, antimicrobial sensitivity, molecular typing and genetic finger printing using RAPD-PCR to determine the genetic finger printing of the recovered strains from humans and companion animals. The prevalence of the MRSA was higher in dog’s swabs than human swabs. Dog swabs showed a rate of (44.4%), cat’s revealed (27.3%), while the owner swabs could detect (42.8%). The antibiotics profiles were 69.2% and all MRSA strains were positive for mecA gene (100%), while only 25 strains (38.5%) were positive for Panton Valentine Leukocidin (PVL gene). Phylogenetic tree revealed 4 clusters with complete genetic relatedness and higher identity between the strains recovered from humans and companion animals. Our results revealed that there is great similarity between the recovered strains, indicating that pets play an important role in colonization and transmitting MRSA to humans, and vice versa.

伴侣动物带有对人类严重危害公共健康的不同微生物；人与伴侣动物之间的友善关系和接触可能是大多数人畜共患细菌（包括耐甲氧西林金黄色葡萄球菌）传播的途径（MRSA）。因此，当前的研究调查了作为MRSA贮藏库的伴侣动物，主要是狗和猫，以及从此类伴侣动物中回收的MRSA菌株与其所有者之间的遗传相似性。在无菌条件下从伴侣动物中收集了一百个拭子，并从接触的受感染者的鼻和软组织中收集了七十个拭子。使用标准的微生物学技术，抗菌敏感性，分子分型和使用RAPD-PCR的遗传指纹对所有样品进行检查，以确定从人类和伴侣动物中回收的菌株的遗传指纹。狗拭子中MRSA的患病率高于人类拭子。狗的拭子检出率为（44.4％），猫的拭子检出率为（27.3％），而所有者的拭子检出率为（42.8％）。mecA基因（100％），而Panton Valentine Leukocidin（PVL基因）只有25株（38.5％）呈阳性。系统发育树揭示出4个簇，它们具有完全的遗传相关性，并且在从人类和伴侣动物中回收的毒株之间具有更高的同一性。我们的结果表明，回收的菌株之间存在很大的相似性，表明宠物在定殖和将MRSA传播给人类方面起着重要作用，反之亦然。