Biological dosimetry of ionizing radiation (IR) exposure relies on validated cytogenetic tests measuring the frequencies of micronuclei (MN) and dicentric chromosomes (DC). IR also causes oxidative damage of biomolecules, including DNA. We evaluated IR-induced genotoxic and oxidative damage in a carefully defined cohort of healthy donors, reducing confounding factors as much as possible. Frequencies of MN and DC (peripheral blood lymphocyte cultures) and oxidative stress parameters (plasma) were quantified. We observed dose dependence of both cytogenetic and biochemical endpoints, independent of age, sex, and smoking habits. Oxidative stress parameters, especially oxidative stress index, malondialdehyde, advanced oxidation protein products, and catalase, may be used confidently to assess IR-induced damage, if cytogenetic results are unavailable.

电离辐射 (IR) 暴露的生物剂量学依赖于经过验证的细胞遗传学测试，可测量微核 (MN) 和双着丝粒染色体 (DC) 的频率。IR 还会引起包括 DNA 在内的生物分子的氧化损伤。我们在精心定义的健康供体队列中评估了 IR 诱导的基因毒性和氧化损伤，尽可能减少混杂因素。MN 和 DC（外周血淋巴细胞培养物）和氧化应激参数（血浆）的频率被量化。我们观察到细胞遗传学和生化终点的剂量依赖性，与年龄、性别和吸烟习惯无关。如果无法获得细胞遗传学结果，可以自信地使用氧化应激参数，尤其是氧化应激指数、丙二醛、高级氧化蛋白产物和过氧化氢酶来评估 IR 诱导的损伤。