Fungal sporulation is affected by many environmental factors, for example, we previously observed that embedding of a hydrophobic polymer net in an agar plate medium significantly accelerates spore formation of some fungi. Here, it was found that the fungal spore formation depended on the surface hydrophobicity of cultivation vessels used for the plate cultivation. In a polypropylene (PP) vessel, six fungal strains produced spores of 1.5 to 514.8 times of those growing in a glass vessel. The contact of vegetative hyphae on the surface of the vessels might trigger the fungal spore formation. Moreover, the spore formation was synergistically accelerated by the reduction of nitrogen source content in an agar plate medium and by the contact to hydrophobic polymers. The synergistic effect depended on the surface area of the hydrophobic polymer. Thus, the combination of the reduction of nitrogen source and the embedding of hydrophobic polymer is expected as a novel and effective procedure for production of fungal spores which are useful for the inoculum in fermentation industry and biocontrol agent in agriculture.

真菌孢子形成受许多环境因素的影响，例如，我们之前观察到，将疏水性聚合物网嵌入琼脂平板培养基中会显着加速某些真菌的孢子形成。在这里，发现真菌孢子的形成取决于用于平板培养的培养容器的表面疏水性。在聚丙烯（PP）容器中，六种真菌菌株产生的孢子是在玻璃容器中生长的孢子的1.5至514.8倍。营养菌丝在血管表面的接触可能会触发真菌孢子的形成。此外，通过减少琼脂平板培养基中氮源的含量以及与疏水性聚合物的接触，协同促进了孢子的形成。协同作用取决于疏水性聚合物的表面积。因此，