Although Saccharomyces cerevisiae and S. cerevisiae var. boulardii share more than 95% genome sequence homology, only S. cerevisiae var. boulardii displays probiotic activity. In this study, the transcriptomic differences exhibited by S. cerevisiae and S. cerevisiae var. boulardii in intestinal like medium were evaluated. S. cerevisiae was found to display stress response overexpression, consistent with higher ability of S. cerevisiae var. boulardii to survive within the human host, while S. cerevisiae var. boulardii exhibited transcriptional patterns associated with probiotic activity, suggesting increased acetate biosynthesis. Resorting to the creation of a S. cerevisiae var. boulardii genomic database within Yeastract+, a possible correlation between loss or gain of transcription factor binding sites in S. cerevisiae var. boulardii promoters and the transcriptomic pattern is discussed. This study suggests that S. cerevisiae var. boulardii probiotic activity, when compared to S. cerevisiae, relies, at least partially, on differential expression regulation, based on promoter variability.

虽然Saccharomyces cerevisiae和S. cerevisiae var. boulardii拥有超过 95% 的基因组序列同源性，只有S. cerevisiae var. boulardii显示益生菌活性。在这项研究中，S. cerevisiae和S. cerevisiae var. 表现出的转录组差异。对肠样培养基中的布拉氏菌进行了评估。发现S. cerevisiae表现出应激反应过度表达，这与S. cerevisiae var.的更高能力一致。boulardii在人类宿主中生存，而S. cerevisiae var。布拉迪表现出与益生菌活性相关的转录模式，表明乙酸盐生物合成增加。诉诸于创造酿酒酵母变种。Yeastract+ 中的boulardii基因组数据库， S. cerevisiae var 中转录因子结合位点的丢失或获得之间可能存在相关性。boulardii启动子和转录组模式进行了讨论。这项研究表明，S. cerevisiae var。与酿酒酵母相比，布拉氏益生菌活性至少部分依赖于基于启动子变异性的差异表达调节。