This study investigated the effects of different levels of dietary selenium (Se) on the growth, antioxidant capacity, hepatopancreas health, and transcriptional-level responses of Litopenaeus vannamei. Sodium selenite was added to basal diet formulations at 0, 0.2, 0.4, and 0.8 mg/kg, with final Se concentrations of 0.13, 0.20, 0.45, and 0.81 mg/kg, respectively. Shrimp were fed the experimental diets for 8 weeks, after which the following parameters were measured: growth performance, antioxidant enzymes activity, ALT, AST activity, endoplasmic reticulum stress and transcriptome. Shrimp fed the 0.45 and 0.81 mg/kg Se diets (P < 0.05) showed the highest weight gain, shrimp fed the 0.13 mg/kg Se diet had the highest hepatosomatic index (P < 0.05), and the endoplasmic reticulum stress related genes were down-regulated (P < 0.05) in shrimp fed the 0.45 mg/kg Se diet. Antioxidant enzyme activity (SOD, CAT, and GPx) was enhanced with the increase in the Se level. The transcriptome results showed that glycine, serine, and threonine metabolism, the pentose phosphate pathway, and ascorbate and aldarate metabolism were enriched in shrimp fed the 0.45 mg/kg Se diet, while focal adhesions, gap junctions, tight junctions, and adhesion junctions were found in shrimp fed the 0.13 and 0.81 mg/kg Se diets. Co-expression analysis showed that genes PINK1 and Innexin (among others), were involved in the influence of Se levels on shrimp. Our results suggested that Se supplementation improved the growth and antioxidant capacity of L. vannamei by promoting the biosynthesis of glutathione and enhancing the activity of antioxidant enzymes. However, high levels of dietary Se can cause endoplasmic reticulum stress and hepatopancreas tissue damage.

这项研究调查了不同水平的饮食硒对硒对虾生长，抗氧化能力，肝胰腺健康和转录水平反应的影响。将亚硒酸钠以0、0.2、0.4和0.8 mg / kg的量添加到基础饮食配方中，最终硒浓度分别为0.13、0.20、0.45和0.81 mg / kg。给虾喂食实验饮食8周，然后测量以下参数：生长性能，抗氧化酶活性，ALT，AST活性，内质网应激和转录组。饲喂0.45和0.81 mg / kg硒饮食的 虾增重最高（P <0.05），饲喂0.13 mg / kg硒饮食的虾具有最大的肝体指数（P <0.05）， 饲喂0.45 mg / kg硒日粮的虾的内质网应激相关基因被下调（P <0.05）。随着硒水平的提高，抗氧化酶活性（SOD，CAT和GPx）增强。转录组结果显示，饲喂0.45 mg / kg硒的虾，甘氨酸，丝氨酸和苏氨酸代谢，磷酸戊糖途径以及抗坏血酸盐和藻酸盐代谢丰富，而粘着斑，缝隙连接，紧密连接和粘连饲喂0.13和0.81 mg / kg硒饮食的虾中发现了这种元素。共表达分析表明基因PINK1和Innexin（尤其是）参与了硒水平对虾的影响。我们的结果表明，硒的添加通过促进谷胱甘肽的生物合成和增强抗氧化酶的活性而改善了南美白对虾的生长和抗氧化能力。但是，饮食中高含量的硒会引起内质网应激和肝胰腺组织损伤。