An integrated system for biodiesel and biogas production for the diatom Chaetoceros muelleri was evaluated. We assessed the suitability of osmotic shock pre-treatment of wet microalgal biomass for lipid extraction and biogas production. Osmotic shock pre-treatment released up to 72% of C. muelleri lipid content. Biogas production of pre-treated and lipid-spent microalgae was evaluated by biomethane potential tests and by operating two continuous anaerobic reactors for 200 days. An improvement of 35% in methane production was observed with the pre-treated microalgae in batch conditions, with a maximum methane yield of 521 mL CH₄ g VS⁻¹. The methane yield in the continuous experiments of the pre-treated microalgae fluctuated between 0.3 and 0.7 L CH₄ g VS⁻¹, with an average of 0.52 L CH₄ g VS⁻¹. No significant differences were observed between the pre-treated microalgae without lipid extraction and lipid-spent microalgae in both batch and continuous conditions. The energy potential for a combined biodiesel-biogas biorefinery has been estimated at 20.5 KJ gVS⁻¹ of harvested microalgae.

对硅藻Chaetoceros muelleri的生物柴油和沼气生产的集成系统进行了评估。我们评估了湿微藻生物质渗透压预处理对脂质提取和沼气生产的适用性。渗透压休克预处理释放高达穆勒氏梭菌脂质含量的72％。通过生物甲烷潜力测试以及通过两个连续的厌氧反应器运行200天来评估预处理的和用过脂质的微藻的沼气产量。在批次条件下，使用预处理的微藻可观察到甲烷产量提高35％，最大甲烷产量为521 mL CH ₄  g VS ^-1。预处理微藻的连续实验中的甲烷产率在0.3至0.7 L CH ₄  g VS ^-1之间波动，平均为0.52 L CH ₄  g VS ^-1。在分批和连续条件下，未进行脂质提取的预处理微藻与消耗脂质的微藻之间均未观察到显着差异。据估计，生物柴油-沼气生物精炼厂的能源潜力为收获的微藻类的20.5 KJ gVS ^-1。