Stem Cell Reviews and Reports ( IF 4.5 ) Pub Date : 2021-01-19 , DOI: 10.1007/s12015-020-10099-2 G Desando 1 , I Bartolotti 1 , L Cattini 2 , M Tschon 3 , L Martini 3 , M Fini 3 , A Schiavinato 4 , C Soranzo 4 , B Grigolo 1
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Gathering a better grasp on the adipose stromal vascular fraction (SVF) is demanding among clinicians for osteoarthritis (OA) care because of its promising but multifaceted clinical outcomes. The aim of this preclinical in vitro study was to test whether the mechanical approach with Hy-Tissue SVF system, a class IIa CE marked device of adipose tissue micro-fragmentation, influences the biological features and functions of SVF. We compared mechanical generated-SVF (mSVF) with the enzymatic generated-SVF (eSVF) by testing cell survival, phenotype, differentiation, and paracrine properties using ELISA assays. Both adipose SVF showed 80% viable cells and enrichment for CD-44 marker. The mSVF product preserved the functions of cell populations within the adipose tissue; however, it displayed lowered nucleated cell recovery and CFU-F than eSVF. As for multipotency, mSVF and eSVF showed similar differentiation commitment for osteochondral lineages. Both adipose SVF exhibited an increased release of VEGF, HGF, IGF-1 and PDGF-bb, involved in pathways mediating osteochondral repair and cell migration. Both mSVF and eSVF also displayed high release for the anti-inflammatory cytokine IL-10. After in vitro culture, supernatants from both mSVF and eSVF groups showed a low release of cytokines except for IL-10, thereby giving evidence of functional changes after culture expansion. In this study, mSVF showed active cell populations in the adipose tissue comparable to eSVF with excellent survival, differentiation and paracrine properties under a new mechanical adipose tissue micro-fragmentation system; thereby suggesting its potential use as a minimally invasive technique for OA treatment.
Graphical abstract
中文翻译：
机械处理脂肪组织用于骨关节炎治疗的潜在体外再生特性的前景
更好地掌握脂肪基质血管分数 (SVF) 对骨关节炎 (OA) 治疗的临床医生来说是一项艰巨的任务，因为它具有前景广阔但具有多方面的临床结果。这项临床前体外研究的目的是测试 Hy-Tissue SVF 系统（一种具有 IIa 类 CE 标记的脂肪组织微破碎装置）的机械方法是否影响 SVF 的生物学特征和功能。我们通过使用 ELISA 检测细胞存活、表型、分化和旁分泌特性，将机械生成的 SVF (mSVF) 与酶促生成的 SVF (eSVF) 进行比较。两种脂肪 SVF 均显示出 80% 的活细胞和 CD-44 标记富集。mSVF 产品保留了脂肪组织内细胞群的功能；然而，它的有核细胞回收率和 CFU-F 低于 eSVF。至于多能性，mSVF 和 eSVF 对骨软骨谱系表现出相似的分化倾向。两种脂肪 SVF 均表现出 VEGF、HGF、IGF-1 和 PDGF-bb 释放增加，参与介导骨软骨修复和细胞迁移的途径。mSVF 和 eSVF 还表现出抗炎细胞因子 IL-10 的高释放。体外培养后，mSVF 和 eSVF 组的上清液显示除 IL-10 外的细胞因子释放量较低，从而提供了培养扩增后功能变化的证据。在这项研究中，mSVF 在新的机械脂肪组织微破碎系统下显示出与 eSVF 相当的脂肪组织中的活跃细胞群，具有出色的存活、分化和旁分泌特性；从而表明其作为 OA 治疗的微创技术的潜在用途。
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