Cryptococcosis is a life-threatening infection caused by Cryptococcus neoformans and C. gattii species complex. In the present study, to understand the molecular epidemiology of 208 clinical isolates of Cryptococcus from different parts of India, multilocus sequence typing (MLST) using ISHAM MLST consensus scheme for C. neoformans/C. gattii species complex was used. MLST analysis yielded a total of 10 Sequence Types (STs)—7 STs for C. neoformans and 3 for C. gattii species complex. The majority of isolates identified as C. neoformans belonged to molecular type VNI with predominant STs 31 and 93. Only 3 isolates of C. gattii species complex were obtained, belonging to ST58 and ST215 of VGI and ST69 of VGIV. Phylogenetic analysis revealed less diversity among the clinical Indian isolates compared to the global MLST database. No association between prevalent STs and HIV status, geographical origin or minimum inhibitory concentration (MIC) could be established.

隐球菌病是由新型隐球菌和C. gattii种复合体引起的危及生命的感染。在本研究中，为了了解来自印度不同地区的 208 株隐球菌临床分离株的分子流行病学，使用 ISHAM MLST 共识方案对新型隐球菌/隐球菌进行多位点序列分型 (MLST) 。使用 gattii物种复合物。MLST 分析总共产生了 10 个序列类型 (ST)——7 个 ST 用于C.neoformans，3 个用于C. gattii物种复合体。大多数被鉴定为新型隐球菌的分离株属于分子型 VNI，主要是 ST 31 和 93。只有 3 株C. gattii获得物种复合体，属于 VGI 的 ST58 和 ST215 和 VGIV 的 ST69。系统发育分析显示，与全球 MLST 数据库相比，临床印度分离株之间的多样性较低。无法确定流行的 ST 与 HIV 状态、地理来源或最低抑菌浓度 (MIC) 之间的关联。