The investigation was undertaken with the aim of studying the effect of individual and tank mix applications of zinc sulphate (ZnSO₄), ferrous sulphate (FeSO₄) and urea on zinc (Zn) and iron (Fe) biofortification of chickpea. A field experiment was conducted in 2015–2016 and 2016–2017 with treatments comprising individual and tank mix applications of ZnSO₄ (0.5%), FeSO₄ (0.5%) and urea (2%) at flowering and pod formation stages along with control (no nutrient application). Tank mix foliar application of ZnSO₄ (0.5%) + FeSO₄ (0.5%) + urea (2%) resulted in similar Zn (44.01 and 43.01 mg Zn kg⁻¹ grain in the first and second year of study) and Fe (71.08 and 73.91 mg Fe kg⁻¹ grain) content as in case of individual application of these nutrients (45.08 and 45.00 mg Zn kg⁻¹ and 71.41 and 74.16 mg Fe kg⁻¹ grain) thus showing a positive impact over unsprayed control (25.08 and 25.53 mg Zn kg⁻¹ and 47.58 and 48.66 mg Fe kg⁻¹ grain). Addition of urea (2%) helped in further improvement of Zn and Fe contents over ZnSO₄ (0.5%) + FeSO₄ (0.5%) spray only. Both tank mix application and individual application of these nutrients recorded significantly higher grain yield and protein content over sole application and control. Tank mix application of ZnSO₄ (0.5%), FeSO₄ (0.5%) and urea (2%) at flowering and pod formation stages of chickpea is a new method of biofortification which improves Zn and Fe contents and needs a special attention for improving malnutrition in humans.

进行这项研究的目的是研究单独和桶装施用硫酸锌（ZnSO ₄），硫酸亚铁（FeSO ₄）和尿素对鹰嘴豆锌（Zn）和铁（Fe）生物强化的影响。在2015–2016年和2016–2017年进行了田间试验，处理包括在开花期和荚果形成阶段分别进行单独和桶混施用的ZnSO ₄（0.5％），FeSO ₄（0.5％）和尿素（2％）以及对照。 （不施营养）。ZnSO ₄（0.5％）+ FeSO ₄（0.5％）+尿素（2％）的桶混叶面施用得到相似的Zn（44.01和43.01 mg Zn kg ^-1谷物在研究）和Fe（71.08和73.91毫克铁的第一和第二年千克^-1粒）中的含量按这些营养素的单个应用程序（45.08和45.00毫克锌公斤的情况下^-1和71.41和74.16毫克铁公斤^{- 1}粒）因此对未喷洒的对照品（25.08和25.53 mg Zn kg ^-1和47.58和48.66 mg Fe kg ^-1颗粒）产生积极影响。与仅喷洒ZnSO ₄（0.5％）+ FeSO ₄（0.5％）喷雾剂相比，添加尿素（2％）有助于进一步提高Zn和Fe含量。与单独施用和控制相比，桶混施用和单独施用这些养分均显示出明显更高的谷物产量和蛋白质含量。ZnSO的桶混应用鹰嘴豆开花和结荚阶段的₄（0.5％），FeSO ₄（0.5％）和尿素（2％）是一种生物强化的新方法，可提高Zn和Fe的含量，并需要特别注意改善人类的营养不良。