This study highlighted the impact of different organic manuring on the decay kinetics and temperature sensitivity (Q 10 ) of three enzymes viz. dehydrogenase (DHA), fluorescein diacetate hydrolase (FDA) and β-glucosidase (GLU) under varying hydrothermal regimes in the subtropical Cambisols in India. We hypothesised that along with organics, water content and temperature will also affect enzyme activities. Soil samples were collected from five treatments, viz., control, 100% nitrogen-phosphorus-potassium (NPK), vermicompost, compost and farmyard manure (FYM)–treated plots in an on-going maize-wheat field experiment, and were incubated at 25 and 35 °C temperatures and 0.033 and 0.1 MPa soil water contents. Activities of all enzymes were found higher in organic-treated plots than NPK-treated plots throughout the incubation period due to higher substrates and energy supply. At constant water content, the increased temperature significantly increased the decay rates of all the enzymes owing to dissolution and diffusion of carbon substrates towards active sites of enzymes. However, at a specified temperature, water stress decreased the decay rates in vermicompost- and compost-treated soils but increased in FYM-treated soils, indicating a significant influence of soil amendments on the decay kinetics due to specific microbial adaptation mechanisms and substrate biochemistry of FYM. The Q 10 values of DHA and FDA increased by ~ 59 and 46%, respectively but that of GLU decreased by ~ 44% with declining water content due to their capacity to degrade diverse substrates in the soils. FYM application could be beneficial for nutrient mineralisation under adverse conditions to support plant nutrient demand.

中文翻译：

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印度亚热带 Cambisols 小麦-玉米种植系统中不同热液条件下施肥对酶的衰变动力学的影响

该研究强调了不同有机肥对三种酶的衰变动力学和温度敏感性 (Q 10 ) 的影响，即。脱氢酶 (DHA)、荧光素二乙酸水解酶 (FDA) 和 β-葡萄糖苷酶 (GLU) 在印度亚热带 Cambisols 的不同热液条件下。我们假设与有机物一样，含水量和温度也会影响酶的活性。从五个处理收集土壤样品，即对照、100% 氮磷钾 (NPK)、蚯蚓堆肥、堆肥和农家肥 (FYM) 在正在进行的玉米-小麦田间试验中处理的地块，并进行孵化在 25 和 35 °C 的温度和 0.033 和 0.1 MPa 的土壤含水量下。由于更高的底物和能量供应，在整个孵育期内，有机处理的地块中所有酶的活性都高于 NPK 处理的地块。在恒定的水含量下，由于碳底物向酶的活性位点溶解和扩散，升高的温度显着增加了所有酶的衰变速率。然而，在特定温度下，水分胁迫降低了蚯蚓堆肥和堆肥处理土壤的腐烂率，但在 FYM 处理的土壤中增加，表明由于特定的微生物适应机制和底物生物化学，土壤改良剂对腐烂动力学有显着影响。 FYM。DHA 和 FDA 的 Q 10 值分别增加了约 59% 和 46%，分别但 GLU 的减少了约 44%，因为它们能够降解土壤中的各种基质，因此随着含水量的下降。FYM 应用可能有利于在不利条件下的养分矿化，以支持植物养分需求。