The present study was designed to unravel the bioevaluation of [^99mTc]Tc-GSH in HT 29 colon cancer cell line as well as in experimentally induced colon cancer. The radiochemical yield was observed to be 95.93 ± 1.09%. In silico docking studies revealed that [^99mTc]Tc-GSH gets well accommodated in the binding pocket of gamma-glutamyl transpeptidase in comparable orientation with lowest estimated binding energy − 81.90. Toxicity assays i.e. 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide and trypan blue exclusion assay showed that radiocomplex is non-toxic to HT 29 cells. In conclusion, in vitro and in vivo results indicated the [^99mTc]Tc-GSH radiocomplex has tumor delineating features and also exhibits selectivity for colon tumor.

本研究的目的是解开[的bioevaluation ^99米锝]锝GSH在HT 29结肠癌细胞系，以及在实验性诱导的结肠癌。观察到放射化学产率为95.93±1.09％。在计算机对接研究中发现，[ ^99m Tc] Tc-GSH可以很好地容纳在γ-谷氨酰转肽酶的结合口袋中，方向相当，估计结合能最低-81.90。毒性测定，即3-（4,5-二甲基噻唑基-2）-2,5-二苯基四唑溴化物和锥虫蓝排除测定表明，放射性复合物对HT 29细胞无毒。总之，体外和体内结果表明[ ^99m Tc] Tc-GSH^放射性复合物具有肿瘤勾画特征，并且还表现出对结肠肿瘤的选择性。